人肝单核细胞

Liver Mononuclear Cells (LMC) are located in the liver and perform vital functions for the innate and adaptive immune system. Primary LMC are a heterogeneous population of small lymphocytes, large granular lymphocytes, monocytes, and granulocytes. Primary human LMC (HLMC) can be utilized to study the innate and adaptive immune system.

HLMC from ScienCell Research Laboratories are isolated from human liver. HLMC are depleted of erythrocytes and hepatic macrophages, cryopreserved immediately after isolation, and delivered frozen. HLMC are a mixed population of cells that include T-lymphocytes, B-lymphocytes, NK-cells, and monocytes. Each vial contains at least 10 million cells in 1 ml volume. HLMC are quality control tested for viability. HLMC are negative for are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HLMC can be maintained for a short period of time in culture using the conditions provided by ScienCell Research Laboratories and are not intended for long-term culture.

Recommended Medium

It is recommended to use HematoGro Medium (HeGM, Cat. #5501) for short-term maintenance of HLMC in vitro.

肝单核细胞（LMC）存在于肝脏中，在先天性免疫和适应性免疫系统中发挥重要作用。原代LMC是由小淋巴细胞、大颗粒淋巴细胞、单核细胞和粒细胞组成的异质性细胞群。原代人肝单核细胞（HLMC）可用于研究先天性免疫和适应性免疫系统。

ScienCell Research Laboratories提供的HLMC分离自人肝脏。HLMC已去除红细胞和肝脏巨噬细胞，在分离后立即进行冻存，并以冷冻状态运输。HLMC为混合细胞群，包括T淋巴细胞、B淋巴细胞、NK细胞和单核细胞。每支冻存管含有至少1000万个细胞，体积为1 ml。HLMC已通过细胞活率质量控制检测。HLMC经检测不含HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌。根据ScienCell Research Laboratories提供的培养条件，HLMC可在培养条件下维持短期培养，不适用于长期培养。

推荐培养基

建议使用HematoGro培养基（HeGM，产品编号5501）对HLMC进行体外短期维持培养。

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