人睾丸间质细胞

Leydig Cells (LC) are located in the interstitium of the testis near the seminiferous tubules. One of the main functions of LC is to produce testosterone after stimulation by pituitary luteinizing hormone. LC, through synthesis of testosterone, play a key role in mammalian sex determination by inducing differentiation of the Wolffian ducts into male reproductive organs. Additionally, LC produce insulin-like growth factor 3 which is required for scrotal descent of the testis. Recent studies have indicated that molecules such as cyclic nucleotide phosphodiesterases can modulate testosterone production in LC, suggesting that testosterone synthesis may be manipulated using pharmacological targeting. Cultured Human LC are a useful in vitro model to better understand testicular development and to develop treatments for male reproductive disorders.

Human Leydig Cells (HLC) from ScienCell Research Laboratories are isolated from human testis. HLC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HLC are characterized by immunofluorescence with antibody specific to GATA-4 and CK-18. HLC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HLC are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, HLC are not recommended for expanding or long-term cultures due to limited expansion capacity.

Recommended Medium

It is recommended to use Leydig Cell Medium (LCM, Cat. #4511) for the culturing of HLC in vitro.

睾丸间质细胞（Leydig 细胞，LC）位于睾丸间质中，邻近曲细精管。LC 的主要功能之一是在垂体黄体生成素（LH）的刺激下产生睾酮。LC 通过合成睾酮，在哺乳动物雄性性别分化过程中发挥重要作用，可促进沃尔夫管向男性生殖器官分化。此外，LC 还能产生胰岛素样生长因子 3（IGF3），该因子对于睾丸下降至阴囊至关重要。近期研究表明，环核苷酸磷酸二酯酶等分子可以调节 LC 的睾酮生成，提示可通过药理学靶向调控睾酮合成。培养的人睾丸间质细胞可作为有价值的体外模型，用于深入研究睾丸发育机制，并探索男性生殖系统疾病的治疗方法。

ScienCell Research Laboratories 的人睾丸间质细胞（HLC）分离自人睾丸组织。HLC 以第 1 代（P1）冻存，并以冷冻状态运输。每瓶含有 >5 × 10⁵ 个细胞，体积为 1 ml。HLC 采用针对 GATA-4 和 CK-18 的特异性抗体进行免疫荧光鉴定。HLC 经检测对 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌均呈阴性。在 ScienCell Research Laboratories 提供的培养条件下，HLC 可进行进一步培养；但由于其扩增能力有限，不建议用于细胞扩增或长期培养。

推荐培养基

推荐使用睾丸间质细胞培养基（Leydig Cell Medium，LCM，产品编号：4511）在体外培养 HLC。

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