人软脑膜周细胞

Central nervous system pericytes are perivascular cells that are closely associated with the endothelium of capillaries and other small vessels. Pericytes, located near endothelial cells, communicate with other cells by extending long cytoplasmic processes which wrap around the capillaries. Pericytes participate in a variety of processes including angiogenesis, endothelial cell survival, regulation of capillary blood flow, and establishment and maintenance of the bloodbrain barrier. Pericyte dysregulation has been linked to several pathological conditions such as hypertension, diabetic retinopathy, atherosclerosis, multiple sclerosis, Alzheimer’s disease, and tumor angiogenesis. The unique and diverse functions of pericytes make them novel candidates for cell therapy in regenerative medicine. Leptomeningeal pericytes (LP) are derived from the leptomeningeal layer surrounding the brain. Cultured human leptomeningeal pericytes (HLP) are a
useful in vitro model for studying a wide variety of central nervous system diseases.

HLP from ScienCell Research Laboratories are isolated from human brain. HLP are cryopreserved at passage one after purification and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HLP are characterized by immunofluorescence with antibody specific to α-smooth muscle actin, platelet derived growth factor receptor-β (PDGFR-β), and NG2. HLP are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HLP are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium:

It is recommended to use Pericyte Medium (PM, Cat. #1201) for culturing HLP in vitro.

中枢神经系统周细胞是一类血管周围细胞，与毛细血管及其他小血管的内皮细胞密切相关。周细胞位于内皮细胞附近，并可通过伸出长的细胞质突起包绕毛细血管，与周围其他细胞进行相互作用。周细胞参与多种生理过程，包括血管生成、内皮细胞存活、毛细血管血流调节以及血脑屏障的形成与维持。周细胞功能失调与多种病理状况有关，包括高血压、糖尿病视网膜病变、动脉粥样硬化、多发性硬化症、阿尔茨海默病和肿瘤血管生成。周细胞具有独特且多样的生物学功能，因此成为再生医学细胞治疗领域备受关注的候选细胞。软脑膜周细胞（LP）来源于包绕脑组织的软脑膜层。培养的人软脑膜周细胞（HLP）可作为一种有价值的体外模型，用于研究多种中枢神经系统疾病。

ScienCell Research Laboratories 的 HLP 分离自人脑，纯化后于第一代（P1）进行冻存，并以冷冻状态提供。每瓶含有 >5 × 10⁵ 个细胞，体积为 1 mL。HLP 通过免疫荧光法，采用针对 α-平滑肌肌动蛋白（α-SMA）、血小板衍生生长因子受体-β（PDGFR-β）和 NG2 的特异性抗体进行表征。HLP 经检测为 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌阴性。在 ScienCell Research Laboratories 提供的培养条件下，HLP 可进一步扩增至少 15 个群体倍增。

推荐培养基：

建议使用周细胞培养基（PM，产品编号 1201）进行 HLP 的体外培养。

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