人晶状体上皮细胞
分离自人晶状体。人晶状体上皮细胞（HLEpiC） 在第一代（P1）时进行冻存，并以冷冻状态提供。每瓶含有 >5 × 105 个细胞，体积为 1 mL。
The mammalian lens consists of two cell types, lens fiber cells which form the bulk of the lens, and a monolayer of epithelial cells that cover the anterior surface of the fibers. Lens epithelial cells are responsible for homeostasis regulation of the lens, including electrolyte and fluid transport. Under normal development, lens epithelial cells progressively differentiate and mature. Lens epithelial cells then migrate from the equatorial region into the interior of the lens to produce transparent crystallins, elongate to form lens fiber cells, and eventually lose their nuclei and other organelles. Studies have shown that lens epithelial cell differentiation and lens polarization are regulated by growth factors present in the ocular fluids, such as epidermal growth factor, basic fibroblast growth factor, insulin growth factor, and insulin.
HLEpiC from ScienCell Research Laboratories are isolated from human lens. HLEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HLEpiC are characterized by immunofluorescence with antibodies specific to cytokeratin18 and/or cytokeratin-19. HLEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HLEpiC are guaranteed to further expand for 5 population doublings under the condition provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Epithelial Cell Medium (EpiCM, Cat. #4101) for culturing HLEpiC in vitro.
哺乳动物晶状体由两类细胞组成：构成晶状体主体的晶状体纤维细胞，以及覆盖在纤维细胞前表面的单层晶状体上皮细胞。晶状体上皮细胞负责维持晶状体的稳态，包括调节电解质和液体的运输。在正常发育过程中，晶状体上皮细胞会逐渐发生分化和成熟。随后，晶状体上皮细胞从赤道部向晶状体内部迁移，产生透明的晶状体蛋白，并逐渐延长形成晶状体纤维细胞，最终失去细胞核及其他细胞器。研究表明，晶状体上皮细胞的分化和极性形成受到眼内液中多种生长因子的调控，包括表皮生长因子（EGF）、碱性成纤维细胞生长因子（bFGF）、胰岛素样生长因子（IGF）和胰岛素等。
ScienCell Research Laboratories 的 HLEpiC 分离自人晶状体，于第一代（P1）进行冻存，并以冷冻状态提供。每瓶含有 >5 × 105 个细胞，体积为 1 mL。HLEpiC 通过免疫荧光法，采用针对细胞角蛋白 18（cytokeratin-18）和/或细胞角蛋白 19（cytokeratin-19）的特异性抗体进行表征。HLEpiC 经检测为 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌阴性。在 ScienCell Research Laboratories 提供的培养条件下，HLEpiC 可进一步扩增至少 5 个群体倍增。
推荐培养基
建议使用上皮细胞培养基（EpiCM，产品编号 4101）进行 HLEpiC 的体外培养。
| 目录编号 | 6550 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | HLEpiC |
| 规格/数量 | 5 x 10^5 cells/vial |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后请立即将细胞从干冰直接转移至液氮中，并在进行细胞培养实验前始终保存在液氮中。 |
| 运输 | 干冰。 |
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the products.
