人虹膜色素上皮细胞
分离自人虹膜。人虹膜色素上皮细胞（HIPEpiC） 在第一代（P1）时进行冻存，并以冷冻状态提供。每瓶含有 >5 × 105 个细胞，体积为 1 mL。
The iris is a pigmented disk with a variable aperture which controls the size of the pupil and the amount of light reaching the retina. It consists of the anterior limiting layer, the stroma, the dilator muscle layer, and the posterior pigmented epithelium. The iris pigment epithelial cells (IPEpiC) share the same embryonic origin as retinal pigment epithelial cells (RPE) and thus exhibit similar functional properties as RPE, including turnover of photoreceptors and synthesis of trophic factors. Previous studies showed that IPEpiC transplanted into the subretinal space inhibited abnormal neovascularization and photoreceptor degeneration, which suggests that IPEpiC transplantation may
be used in the future to treat retinal disorders.
HIPEpiC from ScienCell Research Laboratories are isolated from human iris. HIPEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HIPEpiC are characterized by immunofluorescence with antibodies specific to cytokeratin18 and/or cytokeratin-19. HIPEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HIPEpiC are guaranteed to further expand for 10 population doublings under the condition provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Epithelial Cell Medium (EpiCM, Cat. #4101) for culturing HIPEpiC in vitro.
虹膜是一个具有可变开口的有色圆盘，通过调节瞳孔大小来控制到达视网膜的光线量。虹膜由前界膜、基质层、瞳孔开大肌层和后部色素上皮层组成。虹膜色素上皮细胞（IPEpiC）与视网膜色素上皮细胞（RPE）具有相同的胚胎来源，因此具有相似的功能特性，包括参与光感受器细胞的更新以及营养因子的合成。既往研究表明，将 IPEpiC 移植至视网膜下腔可抑制异常新生血管形成和光感受器细胞变性，这提示未来可将 IPEpiC 移植用于视网膜疾病的治疗。
ScienCell Research Laboratories 的 HIPEpiC 分离自人虹膜，于第一代（P1）进行冻存，并以冷冻状态提供。每瓶含有 >5 × 105 个细胞，体积为 1 mL。HIPEpiC 通过免疫荧光法，采用针对细胞角蛋白 18（cytokeratin-18）和/或细胞角蛋白 19（cytokeratin-19）的特异性抗体进行表征。HIPEpiC 经检测为 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌阴性。在 ScienCell Research Laboratories 提供的培养条件下，HIPEpiC 可进一步扩增至少 10 个群体倍增。
推荐培养基
建议使用上皮细胞培养基（EpiCM，产品编号 4101）进行 HIPEpiC 的体外培养。
| 目录编号 | 6560 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | HIPEpiC |
| 规格/数量 | 5 x 10^5 cells/vial |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后请立即将细胞从干冰直接转移至液氮中，并在进行细胞培养实验前始终保存在液氮中。 |
| 运输 | 干冰。 |
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the products.
