人虹膜成纤维细胞
分离自人虹膜。人虹膜成纤维细胞（HIrF） 在第一代（P1）进行冻存，并以冷冻状态提供。每瓶含有 >5 × 105 个细胞，体积为 1 mL。
The iris is a pigmented disk with a variable aperture which controls the size of the pupil and the amount of light reaching the retina. It consists of the anterior limiting layer, the stroma, the dilator muscle layer, and the posterior pigmented epithelium. Iris fibroblasts (IrF), which are located in the iris, are mesenchymal cells derived from the embryonic mesoderm. The main functions of IrF are to maintain the structural integrity of the connective tissue and to aid in tissue repair and remodeling. Under disease conditions, such as with rubeosis iridis, neovascularization can occur on the anterior surface of the iris and result in fibrosis. If the rubeosis iridis condition is left untreated, a neovascular glaucoma can develop. Patients with diabetes and diabetic retinopathy are at a higher risk of developing rubeosis iridis. Human IrF (HIrF) cultures can be used as an in vitro model for studying fibrosis and associated disorders.
HIrF from ScienCell Research Laboratories are isolated from human iris. HIrF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HIrF are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. HIrF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HIrF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing HIrF in vitro.
虹膜是一个具有可变开口的有色圆盘，通过调节瞳孔大小来控制到达视网膜的光线量。虹膜由前界膜、基质层、瞳孔开大肌层和后部色素上皮层组成。位于虹膜中的虹膜成纤维细胞（IrF）是来源于胚胎中胚层的间充质细胞。IrF 的主要功能是维持结缔组织的结构完整性，并参与组织修复和重塑。在虹膜新生血管等疾病状态下，虹膜前表面可能发生新生血管形成并导致纤维化。如果虹膜新生血管未得到治疗，可能进一步发展为新生血管性青光眼。糖尿病和糖尿病视网膜病变患者发生虹膜新生血管的风险较高。人虹膜成纤维细胞（HIrF）培养物可作为一种体外模型，用于研究纤维化及相关疾病。
ScienCell Research Laboratories 的 人虹膜成纤维细胞（HIrF） 分离自人虹膜，于第一代（P1）进行冻存，并以冷冻状态提供。每瓶含有 >5 × 105 个细胞，体积为 1 mL。HIrF 通过其梭形细胞形态以及采用针对纤连蛋白（fibronectin）的特异性抗体进行免疫荧光染色来表征。HIrF 经检测为 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌阴性。在 ScienCell Research Laboratories 提供的培养条件下，HIrF 可进一步扩增至少 15 个群体倍增。
推荐培养基
建议使用成纤维细胞培养基（FM，产品编号 2301）进行 HIrF 的体外培养。
| 目录编号 | 6610 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | HIrF |
| 规格/数量 | >5 x 10^5 cells in 1 ml volume. |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后请立即将细胞从干冰直接转移至液氮中，并在进行细胞培养实验前始终保存在液氮中。 |
| 运输 | 干冰。 |
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