人肝内胆管上皮细胞

Intrahepatic biliary epithelial cells (IBEpiC) line the intrahepatic biliary tree, a complex threedimensional network of tubular conduits of varying diameters within the liver. They comprise only 3 – 5% of the total population of liver cells, yet produce as much as 40% of the daily output of bile. A number of studies have demonstrated that IBEpiC play an important role in maintaining, modifying and augmenting the composition of canalicular bile by a coordinated series of hormoneregulated secretory and absorptive processes. IBEpiC also have an active immunologic role in both the innate and adaptive immune responses. IBEpiC secrete chemokines and cytokines and serve to localize the immune response by expressing critical cell adhesion molecules. Under basal conditions, IBEpiC are thought to be mitotically dormant, however, IBEpiC proliferate markedly in response to injury. Cultured Human IBEpiC (HIBEpiC) are a useful in vitro model for studying biliary cirrhosis and liver disease.

HIBEpiC from ScienCell Research Laboratories are isolated from human liver tissue. HIBEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HIBEpiC are characterized by immunofluorescence with antibody specific to cytokeratin18 and/or cytokeratin-19. HIBEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HIBEpiC are guaranteed to further expand for 10 population doublings in the condition provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Epithelial Cell Medium (EpiCM, Cat. #4101) for the culturing of HIBEpiC in vitro.

肝内胆管上皮细胞（IBEpiC）衬于肝内胆管树，该结构是肝脏内由不同直径管状结构组成的复杂三维网络。尽管其仅占肝细胞总数的 3–5%，但却可产生每日约 40% 的胆汁分泌量。大量研究表明，IBEpiC 通过一系列由激素调控的分泌与吸收过程，在维持、调节及优化小管胆汁成分方面发挥重要作用。同时，IBEpiC 还参与机体的免疫调控，在先天免疫与适应性免疫反应中均具有活跃作用。它们能够分泌趋化因子和细胞因子，并通过表达关键细胞黏附分子来促进局部免疫反应的定位。正常生理状态下，IBEpiC 被认为处于有丝分裂静止状态，但在损伤发生时可显著增殖。体外培养的人肝内胆管上皮细胞（HIBEpiC）是研究胆汁性肝硬化及肝脏疾病的重要模型。

ScienCell Research Laboratories 提供的 HIBEpiC 分离自人肝组织。细胞在第一代传代后进行冷冻保存，并以冻存形式提供。每瓶含有超过 5 x 10⁵ 个细胞，体积为 1 ml。HIBEpiC 通过针对细胞角蛋白 18 和/或细胞角蛋白 19 的特异性抗体进行免疫荧光鉴定。经检测，细胞不含 HIV-1、HBV、HCV、支原体、细菌、酵母及真菌污染。在 ScienCell Research Laboratories 提供的培养条件下，HIBEpiC 可稳定扩增至少 10 次群体倍增。

推荐培养基

推荐使用 Epithelial Cell Medium（EpiCM，货号 #4101）用于 HIBEpiC 的体外培养。