人肠成纤维细胞

Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies, as they are one of the easiest types of cells to grow in culture. In addition, their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is evidence showing that fibroblasts in various organs are intrinsically different. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen. They are responsible for much of the synthesis of extracellular matrix in connective tissues and play a central role in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types in tissues. For example, the development of bowel stenosis in Crohn’s disease patients is caused by extreme fibroblast proliferation and extracellular matrix expansion.

HIF from ScienCell Research Laboratories are isolated from human intestinal tissue. HIF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HIF are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. HIF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HIF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing HIF in vitro.

成纤维细胞是来源于胚胎中胚层的间充质细胞，已广泛应用于各种细胞和分子生物学研究，因为其易于在体外培养。此外，成纤维细胞具有较强的耐受性，可适用于多种实验操作，包括基因转染和显微注射等研究。研究表明，不同器官中的成纤维细胞在生物学特性上存在内在差异。成纤维细胞可分泌富含 I 型和/或 III 型胶原蛋白的非刚性细胞外基质，是结缔组织细胞外基质合成的重要来源，并在伤口愈合过程中发挥核心作用。许多疾病与成纤维细胞密切相关，其原因可能是成纤维细胞参与疾病的病因机制，也可能是其他组织细胞受损后伴随发生的纤维化。例如，克罗恩病患者肠道狭窄的形成与成纤维细胞的过度增殖及细胞外基质扩增有关。

ScienCell Research Laboratories 提供的人肠成纤维细胞（HIF）分离自人肠道组织。HIF 在第一代（P1）阶段进行冻存，并以冷冻形式运输。每瓶含有超过 5 × 10⁵ 个细胞，总体积为 1 ml。HIF 通过其梭形细胞形态以及使用针对纤连蛋白（fibronectin）的特异性抗体进行免疫荧光染色鉴定。HIF 经检测不含 HIV-1、HBV、HCV、支原体、细菌、酵母菌及真菌。在 ScienCell Research Laboratories 提供的培养条件下，HIF 可保证进一步扩增至少 15 次群体倍增。

推荐培养基

建议使用成纤维细胞培养基（Fibroblast Medium，FM，产品编号 #2301）对 HIF 进行体外培养。

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