人胃成纤维细胞

Gastric fibroblasts (GF), which are located in the stomach, are mesenchymal cells derived from the embryonic mesoderm. Fibroblasts secrete a non-rigid extracellular matrix that is both rich in type I and/or type III collagen. They are responsible for much of the synthesis of extracellular matrix in connective tissues and play a central role in wound healing. Studies in vitro and in vivo show that GF can regulate epithelial regeneration by influencing epithelial cell proliferation and differentiation. In addition to their role in wound healing, human GF (HGF) have been shown to be key contributors in the development and progression of gastric carcinoma. HGF are a useful model for examining the underlying mechanisms that cause gastric carcinoma and may provide novel insights for developing new cancer drug therapies.

HGF from ScienCell Research Laboratories are isolated from human stomach tissue. HGF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HGF are characterized by immunofluorescence with antibody specific to fibronectin. HGF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HGF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research
Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing HGF in vitro.

胃成纤维细胞（GF）位于胃组织中，是来源于胚胎中胚层的间充质细胞。成纤维细胞可分泌富含 I 型和/或 III 型胶原蛋白的柔性细胞外基质，在结缔组织细胞外基质的合成中发挥重要作用，并在伤口愈合过程中发挥核心作用。体外和体内研究表明，GF 可通过调节上皮细胞的增殖和分化参与上皮组织再生。除参与伤口愈合外，研究还表明，人胃成纤维细胞（HGF）在胃癌的发生和进展过程中发挥重要作用。HGF 可作为研究胃癌发生机制的有用模型，并有望为开发新型抗癌药物提供新的研究思路。

ScienCell Research Laboratories 提供的人胃成纤维细胞（HGF）分离自人胃组织。HGF 在第一代（P1）阶段进行冻存，并以冷冻形式运输。每瓶含有超过 5 × 10⁵ 个细胞，总体积为 1 ml。HGF 通过使用针对纤连蛋白（fibronectin）的特异性抗体进行免疫荧光染色鉴定。HGF 经检测不含 HIV-1、HBV、HCV、支原体、细菌、酵母菌及真菌。在 ScienCell Research Laboratories 提供的培养条件下，HGF 可保证进一步扩增至少 15 次群体倍增。

推荐培养基

建议使用成纤维细胞培养基（Fibroblast Medium，FM，产品编号 #2301）对 HGF 进行体外培养。