人胆囊成纤维细胞

Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies, as they are one of the easiest types of cells to grow in culture. Their durability makes them amenable to a variety of manipulations ranging from gene transfection to microinjection. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen. They are responsible for synthesis of extracellular matrix in connective tissue and play a central role in wound healing. Human gallbladder fibroblasts (HGBF) are responsible for eicosanoid synthesis, specifically PGI2. Chronic stimulation of HGBF results in up-regulated synthesis and release of PGI2, which aids in formation of early acute cholecystitis. HGBF are very versatile cells and could be used to study many different conditions, including early stages of acute cholecystitis.

HGBF from ScienCell Research Laboratories are isolated from human gallbladder tissue. HGBF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HGBF are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. HGBF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HGBF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for the culturing of HGBF in vitro.

成纤维细胞是一类来源于胚胎中胚层的间充质细胞。由于其易于体外培养、生长稳定，因此被广泛应用于各类细胞与分子生物学研究。成纤维细胞具有较强的耐受性，适用于多种实验操作，包括基因转染、显微注射等。该类细胞能够分泌富含 I 型和/或 III 型胶原蛋白的非刚性细胞外基质，在结缔组织细胞外基质的合成以及创伤修复过程中发挥重要作用。人胆囊成纤维细胞（HGBF）参与类二十烷酸的合成，尤其与前列环素 PGI2 的生成密切相关。持续刺激 HGBF 可导致 PGI2 的合成与释放上调，从而促进早期急性胆囊炎的形成。由于其良好的研究应用价值，HGBF 可用于多种疾病相关研究，包括急性胆囊炎早期阶段的机制研究。

ScienCell Research Laboratories 提供的人胆囊成纤维细胞（HGBF）分离自人胆囊组织。细胞于第一代传代时进行冷冻保存，并以冻存形式提供。每瓶含有超过 5 x 10⁵ 个细胞，总体积为 1 ml。HGBF 具有典型的梭形成纤维细胞形态，并经针对纤维连接蛋白（fibronectin）的特异性抗体进行免疫荧光鉴定。经检测，细胞不含 HIV-1、HBV、HCV、支原体、细菌、酵母及真菌污染。在 ScienCell Research Laboratories 推荐的培养条件下，HGBF 可稳定扩增至少 15 次群体倍增。

推荐培养基

推荐使用 Fibroblast Medium（FM，货号 #2301）用于 HGBF 的体外培养。