人食管上皮细胞

The human esophagus is lined by a non-keratinizing, stratified squamous epithelium whose apical cell membranes and intercellular junctional complexes form a barrier against the influx of luminal content. The barrier helps to reduce exposure of surface cells to changes in osmolality, which occurs frequently within the esophageal lumen. Histologically, the esophageal epithelium consists of two zones, the basal and differentiated zones. Cellular proliferation is limited to the basal zone, and the cells are thought to migrate from this area towards the esophageal lumen. Migration is associated with the initiation of differentiation and the sequential expression of differentiation markers. Human esophageal epithelial cells (HEsEpiC) are an excellent in vitro model to study esophageal epithelium physiology and the mechanisms of esophageal carcinogenesis.

HEsEpiC from ScienCell Research Laboratories are isolated from the human esophagus. HEsEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HEsEpiC are characterized by immunofluorescence with antibody specific to cytokeratin-18 and/or cytokeratin-19. HEsEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HEsEpiC can be further cultured under the conditions provided by ScienCell Research Laboratories; however, HEsEpiC are not recommended for expanding or long-term cultures due to limited expansion capacity.

Recommended Medium

It is recommended to use Epithelial Cell Medium-2 (EpiCM-2, Cat. #4121) for culturing HEsEpiC in vitro.

人食管内壁由非角化复层鳞状上皮构成，其表层细胞膜及细胞间连接复合体形成一道屏障，可防止管腔内容物进入组织内部。该屏障有助于降低表层细胞受到渗透压变化的影响，而食管腔内经常会发生此类渗透压变化。从组织学角度来看，食管上皮由两个区域组成：基底层和分化层。细胞增殖主要发生于基底层，随后细胞被认为会从该区域向食管腔方向迁移。细胞迁移伴随着分化过程的启动以及分化标志物的依次表达。人食管上皮细胞（HEsEpiC）是研究食管上皮生理功能及食管癌发生机制的理想体外研究模型。

ScienCell Research Laboratories 提供的人食管上皮细胞（HEsEpiC）分离自人食管组织。HEsEpiC 在第一代（P1）阶段进行冻存，并以冷冻形式运输。每瓶含有超过 5 × 10⁵ 个细胞，总体积为 1 ml。HEsEpiC 通过使用针对细胞角蛋白-18（cytokeratin-18）和/或细胞角蛋白-19（cytokeratin-19）的特异性抗体进行免疫荧光染色鉴定。HEsEpiC 经检测不含 HIV-1、HBV、HCV、支原体、细菌、酵母菌及真菌。HEsEpiC 可在 ScienCell Research Laboratories 提供的培养条件下进一步培养；但由于其扩增能力有限，不建议用于大量扩增或长期培养。

推荐培养基

建议使用上皮细胞培养基-2（Epithelial Cell Medium-2，EpiCM-2，产品编号 #4121）对 HEsEpiC 进行体外培养。