人硬脑膜成纤维细胞

The dura mater is the outermost layer of the meninges and functions to carry blood from the brain to the heart and contain the cerebrospinal fluid. The dura mater is composed of two layers known as the periosteal and meningeal layer. In general, fibroblasts are responsible for extracellular matrix synthesis in connective tissue and play a major role in wound healing. Dural fibroblasts (DuF) produce extracellar matrix proteins such as collagen and fibronectin. Recent studies have shown that dural fibroblasts have been implicated in activating and sensitizing dural afferents and thereby may contribute to headache pathophysiology. Human DuF (HDuF) are a useful in vitro model to study subdermal hematomas and headache pathophysiology.

HDuF from ScienCell Research Laboratories are isolated from human dura mater. HDuF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HDuF are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. HDuF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HDuF are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing HDuF in vitro.

硬脑膜是脑膜最外层，主要负责将血液从脑部回流至心脏，并参与脑脊液的容纳。硬脑膜由两层组成，分别为骨膜层和脑膜层。成纤维细胞主要负责结缔组织中细胞外基质的合成，并在组织损伤修复过程中发挥重要作用。硬脑膜成纤维细胞（DuF）可产生胶原蛋白、纤连蛋白等细胞外基质蛋白。近期研究表明，硬脑膜成纤维细胞参与硬脑膜传入神经的激活和敏化，因此可能与头痛的病理生理过程有关。人硬脑膜成纤维细胞（HDuF）可作为研究硬膜下血肿及头痛病理生理机制的体外模型。

ScienCell Research Laboratories 的 HDuF 分离自人硬脑膜，冻存于第一代（P1），并以冷冻状态提供。每瓶含有 >5 × 10⁵ 个细胞，体积为 1 mL。HDuF 根据其梭形细胞形态，并通过使用纤连蛋白特异性抗体进行免疫荧光染色来进行表征。HDuF 经检测为 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌阴性。在 ScienCell Research Laboratories 提供的培养条件下，HDuF 可进一步扩增至少 10 个群体倍增。

推荐培养基

建议使用成纤维细胞培养基（FM，产品编号 2301）进行 HDuF 的体外培养。

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