人结膜上皮细胞
分离自人结膜。人结膜上皮细胞（HConEpiC） 在第一代（P1）进行冻存，并以冷冻状态提供。每瓶含有 >5 × 105 个细胞，体积为 1 mL。
The conjunctiva is a translucent membrane that lines the inner surface of the eyelids and covers the sclera. It is overlaid by two layers of stratified, non-keratinized conjunctival epithelial cells (ConEpiC). ConEpiC can be distinguished from the corneal epithelium by a specific expression profile of keratins. ConEpiC is covered by mucin-rich glycocalyx, which promotes tear adherence, prevents pathogen penetrance, and provides lubrication. Decrease or loss of mucin/glycocalyx production generates squamous metaplasia, which may lead to dry eye and ocular surface diseases. ConEpiC also respond to histamine by stimulating phosphatidylinositol turnover and secreting cytokines, and as a result may be a good target when developing topical ocular drugs to treat allergic conjunctivitis.
HConEpiC from ScienCell Research Laboratories are isolated from human conjunctiva. HConEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HConEpiC are characterized by their cobble stone morphology in serum-free culture medium and immunofluorescence with antibodies specific to cytokeratin-18 and/or cytokeratin-19. HConEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HConEpiC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Corneal Epithelial Cell Medium (CEpiCM, Cat. #6511) for culturing HConEpiC in vitro.
结膜是一种半透明的膜，覆盖眼睑内表面并覆盖巩膜。其表面覆盖着两层复层非角化结膜上皮细胞（ConEpiC）。ConEpiC 可通过角蛋白的特异性表达谱与角膜上皮细胞进行区分。ConEpiC 表面覆盖富含黏蛋白的糖萼，可促进泪液附着、防止病原体侵入并提供润滑作用。黏蛋白/糖萼的产生减少或丧失会导致鳞状上皮化生，并可能引发干眼和眼表疾病。ConEpiC 还可对组胺产生反应，通过刺激磷脂酰肌醇代谢并分泌细胞因子发挥作用，因此在开发用于治疗过敏性结膜炎的局部眼科药物时，可能成为一个良好的靶点。
ScienCell Research Laboratories 的 人结膜上皮细胞（HConEpiC） 分离自人结膜，于第一代（P1）进行冻存，并以冷冻状态提供。每瓶含有 >5 × 105 个细胞，体积为 1 mL。HConEpiC 在无血清培养基中通过其鹅卵石样细胞形态以及采用针对细胞角蛋白 18（cytokeratin-18）和/或细胞角蛋白 19（cytokeratin-19）的特异性抗体进行免疫荧光染色来表征。HConEpiC 经检测为 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌阴性。在 ScienCell Research Laboratories 提供的培养条件下，HConEpiC 可进一步扩增至少 10 个群体倍增。
推荐培养基
建议使用角膜上皮细胞培养基（CEpiCM，产品编号 6511）进行 HConEpiC 的体外培养。
| 目录编号 | 6630 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | HConEpiC |
| 规格/数量 | 5 x 10^5 cells/vial |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后请立即将细胞从干冰直接转移至液氮中，并在进行细胞培养实验前始终保存在液氮中。 |
| 运输 | 干冰。 |
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the products.
