人结肠成纤维细胞

The human colon, which is part of the large intestine is located in the lower gastrointestinal tract. The colon functions to remove water, nutrients, and electrolytes from the partially digested food material. Colonic fibroblasts (CoF) are important structural components of the colon and are implicated in a number of diseases including ulcerative colitis, Crohn’s disease, and colon cancer. The main functions of fibroblasts are to maintain the structural integrity of connective tissue and to aid in tissue repair and remodeling. CoF play a central role in wound healing, however, they can also become chronically active during inflammation of the colon. CoF can also affect the homeostasis of the colonic mucosa. During wound healing or during tumor development, fibroblasts can become activated and switch to a myofibroblast morphology and synthesize increased levels of extracellular matrix. The mechanisms that lead to this activation are not well understood, therefore, primary Human (HCoF) can be used to further study the pathophysiology of ulcerative colitis, Crohn’s disease, and colon cancer.

HCoF from ScienCell Research Laboratories are isolated from human colon. HCoF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HCoF are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. HCoF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HCoF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing HCoF in vitro.

人结肠属于大肠的一部分，位于下消化道，主要负责从部分消化的食物残渣中吸收水分、营养物质和电解质。结肠成纤维细胞（CoF）是结肠组织的重要结构组成细胞，与多种疾病的发生和发展密切相关，包括溃疡性结肠炎、克罗恩病和结肠癌。成纤维细胞的主要功能是维持结缔组织的结构完整性，并参与组织修复与重塑。CoF 在伤口愈合过程中发挥核心作用，但在结肠炎症过程中也可能持续处于活化状态。此外，CoF 还可参与调节结肠黏膜的稳态。在伤口愈合或肿瘤发生过程中，成纤维细胞可被激活并转变为肌成纤维细胞形态，同时合成更多细胞外基质。由于导致这一活化过程的具体机制尚未完全阐明，原代人结肠成纤维细胞（HCoF）可用于进一步研究溃疡性结肠炎、克罗恩病和结肠癌的病理生理机制。

ScienCell Research Laboratories 提供的人结肠成纤维细胞（HCoF）分离自人结肠组织。HCoF 在第一代（P1）阶段进行冻存，并以冷冻形式运输。每瓶含有超过 5 × 10⁵ 个细胞，总体积为 1 ml。HCoF 通过其梭形细胞形态以及使用针对纤连蛋白（fibronectin）的特异性抗体进行免疫荧光染色鉴定。HCoF 经检测不含 HIV-1、HBV、HCV、支原体、细菌、酵母菌及真菌。在 ScienCell Research Laboratories 提供的培养条件下，HCoF 可保证进一步扩增至少 15 次群体倍增。

推荐培养基

建议使用成纤维细胞培养基（Fibroblast Medium，FM，产品编号 #2301）对 HCoF 进行体外培养。