成人人支气管上皮细胞

The respiratory epithelium is composed of a mixed population of ciliated, nonciliated, and mucoussecreting cells from proximal to distal airways. The individual characteristics of these cells create not only an effective physical barrier against various noxious substances, but also a highly sophisticated host defense system by producing and releasing a large number of chemical mediators and cytokines. The bronchial epithelium consists of the surface epithelial cells and mucus glands. The surface epithelial cells are made up of three principle cell types: basal, goblet, and ciliated cells, of which the latter two form a suprabasal columnar structure and are necessary for mucociliary clearance. Studies using bronchial epithelial cells (BEpiC) have demonstrated that IL-4 and IL-13 stimulation can modify cellular proliferation, ciliary beating, and mucous production. BEpiC proliferation is also regulated in part by EGF receptor signaling. Cultured BEpiC are a useful in vitro model for studying the function and pathophysiology of the bronchial epithelium.

HBEpiC-a from ScienCell Research Laboratories are isolated from adult human bronchi. HBEpiC-a are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HBEpiC-a are characterized by immunofluorescence with antibodies specific to cytokeratin-18 and/or cytokeratin-19. HBEpiC-a are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBEpiC-a are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Bronchial Epithelial Cell Medium (BEpiCM, Cat. #3211) for culturing HBEpiC-a in vitro.

呼吸道上皮由分布于近端至远端气道的纤毛细胞、非纤毛细胞及黏液分泌细胞等多种细胞组成。这些细胞不仅形成抵御各种有害物质的有效物理屏障，还可通过产生和释放多种化学介质及细胞因子，构建高度完善的宿主防御系统。支气管上皮主要由表面上皮细胞和黏液腺组成，其中表面上皮包括三种主要细胞类型：基底细胞、杯状细胞和纤毛细胞。后两者形成柱状上皮结构，并对黏液纤毛清除功能至关重要。研究表明，白细胞介素-4（IL-4）和白细胞介素-13（IL-13）刺激可调节支气管上皮细胞（BEpiC）的增殖、纤毛摆动及黏液分泌；表皮生长因子（EGF）受体信号通路也参与调控其增殖。因此，培养的 BEpiC 是研究支气管上皮功能及其病理生理机制的重要 in vitro 模型。

ScienCell Research Laboratories 提供的成人人支气管上皮细胞（HBEpiC-a）分离自成人人支气管组织。HBEpiC-a 于第 1 代（P1）进行冻存，并以冻存形式提供。每瓶含有 >5 × 10⁵ 个细胞，悬浮于 1 ml 冻存液中。HBEpiC-a 经细胞角蛋白 18（Cytokeratin-18）和/或细胞角蛋白 19（Cytokeratin-19）特异性抗体免疫荧光鉴定。经检测，HBEpiC-a 不含 HIV-1、HBV、HCV、支原体、细菌、酵母及真菌污染。ScienCell 保证 HBEpiC-a 在推荐培养条件下可继续扩增至少 15 个群体倍增。

推荐培养基

建议使用支气管上皮细胞培养基（Bronchial Epithelial Cell Medium，BEpiCM，产品编号：3211）进行 in vitro HBEpiC-a 培养。