人羊膜上皮细胞
分离自人羊膜。人羊膜上皮细胞（HAEpiC） 在第一代（P1）进行冻存，并以冷冻状态提供。每瓶含有 >5 × 105 个细胞，体积为 1 mL。
Human amniotic membrane is composed of an epithelial cell layer, a basement membrane and an avascular matrix. The amniotic epithelial cells (AEC) are formed from epiblasts on the 8th day after fertilization. As a result of their embryonic origin, AEC lack major histocompatibility complex antigens and have been used for allotransplantation to treat patients with lysosomal diseases. Studies have shown that AEC have multiple functions such as synthesis and release of acetylcholine and catecholamine. In addition, AEC express mRNA coding for dopamine receptors and dopamine transporters. They also express neuronal and glial cell markers, produce basic fibroblast growth factor, hepatocyte growth factor, and transforming growth factor-beta. Human AEC has been suggested as an appropriate human cell model for studying dopamine release and uptake processes, receptor signal transduction and exploring newly developed drugs acting at these receptors.
HAEpiC from ScienCell Research Laboratories are isolated from human amniotic membranes. HAEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HAEpiC are characterized by immunofluorescence with antibodies specific to cytokeratin-18 and/or cytokeratin-19. HAEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HAEpiC are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Epithelial Cell Medium (EpiCM, Cat. #4101) for the culturing of HAEpiC in vitro.
人羊膜由上皮细胞层、基底膜和无血管基质组成。羊膜上皮细胞（AEC）于受精后第 8 天由上胚层细胞形成。由于其胚胎来源，AEC 缺乏主要组织相容性复合体抗原，因此已被用于异体移植，以治疗溶酶体疾病患者。研究表明，AEC 具有多种功能，例如合成和释放乙酰胆碱和儿茶酚胺。此外，AEC 表达编码多巴胺受体和多巴胺转运体的 mRNA。AEC 还表达神经元和胶质细胞标志物，并可产生碱性成纤维细胞生长因子、肝细胞生长因子和转化生长因子-β。人 AEC 被认为是一种适用于研究多巴胺释放和摄取过程、受体信号转导以及探索作用于这些受体的新型药物的人源细胞模型。
ScienCell Research Laboratories 的 人羊膜上皮细胞（HAEpiC） 分离自人羊膜，于第一代（P1）进行冻存，并以冷冻状态提供。每瓶含有 >5 × 105 个细胞，体积为 1 mL。HAEpiC 通过免疫荧光法，采用针对细胞角蛋白 18（cytokeratin-18）和/或细胞角蛋白 19（cytokeratin-19）的特异性抗体进行表征。HAEpiC 经检测为 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌阴性。在 ScienCell Research Laboratories 提供的培养条件下，HAEpiC 可进一步进行培养。
推荐培养基
建议使用上皮细胞培养基（EpiCM，产品编号 4101）进行 HAEpiC 的体外培养。
| 目录编号 | 7110 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | HAEpiC |
| 规格/数量 | 5 x 10^5 cells/vial |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后请立即将细胞从干冰直接转移至液氮中，并在进行细胞培养实验前始终保存在液氮中。 |
| 运输 | 干冰。 |
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