人类年龄相关 ELOVL2 启动子甲基化定量 qPCR 检测试剂盒

DNA methylation at CpG islands in promoters regulates gene expression. It is a dynamic epigenetic process affected by various genetic and environmental factors including aging. The CpG island methylation level at human ELOVL2 gene promoter has been well-associated with chronological age in various populations, cell types, and tissues. ELOVL2 promoter methylation level is therefore a potential marker for human chronological age. ScienCell's Human Age-associated ELOVL2 Promoter Methylation Quantification qPCR Assay Kit (HAEPM) is designed to quantify the level of human ELOVL2 promoter methylation. The reference DNA sample consists of a 1:1 ratio of methylated ELOVL2 promoter copies to non-methylated ELOVL2 promotor copies (both copies represent bisulfite converted promoter sequences), and serves as a reference for calculating the ratio of methylated to non-methylated ELOVL2 promotor of target samples. The carefully designed primers ensure: (i) high efficiency for trustworthy quantification; and (ii) negligible non-specific amplification. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis for amplification specificity and by template serial dilution for amplification efficiency.

DNA 甲基化（发生于启动子区 CpG 岛）在基因表达调控中起重要作用。这是一种动态的表观遗传过程，会受到多种遗传和环境因素（包括衰老）的影响。研究表明，人类 ELOVL2 基因启动子区域的 CpG 岛甲基化水平在不同人群、细胞类型和组织中均与实际年龄高度相关。因此，ELOVL2 启动子甲基化水平可作为评估人类实际年龄的潜在生物标志物。 ScienCell 人类年龄相关 ELOVL2 启动子甲基化定量 qPCR 检测试剂盒（HAEPM）用于定量检测人类 ELOVL2 启动子甲基化水平。该试剂盒中的参考DNA样本由甲基化与非甲基化的 ELOVL2 启动子拷贝按 1:1 比例组成（两者均为经亚硫酸氢盐转化后的启动子序列），可作为标准用于计算目标样品中甲基化与非甲基化 ELOVL2 启动子的比例。 精心设计的引物可确保：（i）高扩增效率，从而获得可靠的定量结果；（ii）极低的非特异性扩增。每组引物均通过 qPCR 熔解曲线分析和凝胶电泳验证其扩增特异性，并通过模板梯度稀释实验验证其扩增效率。