人源肾上腺成纤维细胞

The adrenal gland plays an essential role in regulating homeostasis in the body through the secretion of corticosteroid and androgen hormones. Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies, as they are one of the easiest types of cells to grow in culture. Their durability makes them amenable to a variety of manipulations ranging from gene transfection to microinjection. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen. They are responsible for much of the synthesis of extracellular matrix in connective tissues and play a central role in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types in tissues. For example, the development of bowel stenosis in Crohn’s disease patients is caused by extreme fibroblast proliferation and extracellular matrix expansion.

HAdF from ScienCell Research Laboratories are isolated from human adrenal tissue. HAdF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HAdF are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. HAdF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HAdF are guaranteed to further expand for 15 population doublings under the
conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. No. 2301) for the culturing of HAdF in vitro

肾上腺通过分泌糖皮质激素和雄激素，在维持机体稳态调节过程中发挥重要作用。成纤维细胞是来源于胚胎中胚层的间充质细胞。由于成纤维细胞是最容易进行体外培养的细胞类型之一，因此被广泛应用于各种细胞和分子生物学研究。其较强的耐受性使其适用于多种实验操作，包括基因转染和显微注射等。成纤维细胞可分泌富含Ⅰ型和/或Ⅲ型胶原蛋白的非刚性细胞外基质。它们负责结缔组织中大量细胞外基质的合成，并在创伤愈合过程中发挥核心作用。许多疾病均与成纤维细胞相关，这些关联可能源于成纤维细胞参与疾病发生机制，或由于其他组织细胞损伤后伴随发生的纤维化过程。例如，克罗恩病患者肠道狭窄的形成与成纤维细胞过度增殖以及细胞外基质扩张有关。

ScienCell Research Laboratories 提供的人源肾上腺成纤维细胞（HAdF）分离自人源肾上腺组织。HAdF 以第 1 代细胞进行冻存，并以冷冻状态运输。每瓶含有超过 5 x 10⁵ 个细胞，体积为 1 ml。HAdF 根据其梭形细胞形态以及采用针对纤连蛋白的特异性抗体进行免疫荧光鉴定。HAdF 经检测为 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌阴性。根据 ScienCell Research Laboratories 提供的培养条件，HAdF 可保证进一步扩增 15 个群体倍增周期。

推荐培养基

建议使用成纤维细胞培养基（FM，产品编号 2301）对 HAdF 进行体外培养。

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