人源肾上腺皮质细胞

The adrenal cortex makes up the perimeter of the adrenal gland and plays an essential role in regulating homeostasis in the body through the secretion of corticosteroid and androgen hormones. The secreted steroids arise from the three zones that form the adrenal gland and these zones provide the framework for the adrenal cortex. The cells in the cortex stem from the mesoderm and form three concentric zones: the zona glomerulosa, zona fasciculate, and zona reticularis. Studies have shown that there is extensive interaction between the cortical and medulla regions of the adrenal gland, with cortical cells located within the adrenal medulla and chromaffin cells located within the adrenal cortex. The close contact of these two cell types implies intercellular exchange and allows for further studies of paracrine signaling between the two adrenal endocrine systems. Adrenal cortical cells can be used for further research of hormonal regulation and steroidogenesis.

HAdCC from ScienCell Research Laboratories are isolated from human adrenal cortical tissue. HAdCC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HAdCC are characterized by immunofluorescence with antibodies specific to GFAP. HAdCC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HAdCC are guaranteed to further culture for 15 population doublings under the conditions provided by
ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Mesenchymal Stem Cell Medium (MSCM, Cat. #7501) for the culturing of HAdCC in vitro.

肾上腺皮质构成肾上腺的外周区域，通过分泌糖皮质激素和雄激素，在维持机体稳态调节过程中发挥重要作用。所分泌的类固醇激素来源于构成肾上腺的三个区域，而这些区域共同构成了肾上腺皮质的结构基础。肾上腺皮质细胞来源于中胚层，并形成三个同心区域：球状带（zona glomerulosa）、束状带（zona fasciculata）和网状带（zona reticularis）。研究表明，肾上腺皮质区域与髓质区域之间存在广泛的相互作用，包括位于肾上腺髓质中的皮质细胞以及位于肾上腺皮质中的嗜铬细胞。这两类细胞之间的紧密接触表明存在细胞间物质交换，并为进一步研究两个肾上腺内分泌系统之间的旁分泌信号调控提供了基础。肾上腺皮质细胞可用于激素调控和类固醇生成过程的深入研究。

ScienCell Research Laboratories 提供的人源肾上腺皮质细胞（HAdCC）分离自人源肾上腺皮质组织。HAdCC 以第 1 代细胞进行冻存，并以冷冻状态运输。每瓶含有超过 5 x 10⁵ 个细胞，体积为 1 ml。HAdCC 经采用针对 GFAP 的特异性抗体进行免疫荧光鉴定。HAdCC 经检测为 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌阴性。根据 ScienCell Research Laboratories 提供的培养条件，HAdCC 可保证进一步培养 15 个群体倍增周期。

推荐培养基

建议使用间充质干细胞培养基（MSCM，产品编号 #7501）对 HAdCC 进行体外培养。