人多能干细胞 (hPSC) 来源的心肌细胞 (H9-CM)

Human primary cardiomyocytes and cardiac tissue are superior modeling systems for heart disease studies, drug discovery and toxicity testing compared to animal cells or immortalized cell lines. Human adult primary cardiomyocytes are difficult to obtain due to limited availability of cardiac tissue and they do not typically proliferate in culture. Cardiomyocytes derived from human pluripotent stem cells (HPSC) can potentially provide an unlimited supply of cardiomyocytes for drug screening, toxicity testing and developmental studies.

HPSC-derived Cardiomyocytes (H9-CM) from ScienCell Research Laboratories are differentiated from a pluripotent stem cell line (H9) and enriched for contracting cells. Through the temporal modulation of canonical Wnt signaling and growth factor induction, monolayer-cultured HPSCs are differentiated to contracting cardiomyocytes under serum-free and feeder-free conditions at high efficiency. H9-CM are cryopreserved at passage one after enrichment and delivered frozen. Each vial contains >1 x 10⁶ cells in 1 ml volume. Cells are characterized by immunofluorescence with antibodies specific to α-Actinin and Sarcomeric Tropomyosin. H9-CM are negative for mycoplasma, bacteria, yeast and fungi. H9-CM are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories. H9-CM are not recommended for expanding since the cells show limited proliferation capability in culture.

Recommended Medium

It is recommended to use Cardiac Myocyte Medium (CMM, Cat. #6201) for the culturing of H9-CM in vitro.

与动物细胞或永生化细胞系相比，人源原代心肌细胞及心脏组织是研究心脏疾病、药物筛选和毒性评估的更优模型体系。然而，由于心脏组织来源有限，人源成体原代心肌细胞获取困难，且在体外培养中通常不具备增殖能力。由人多能干细胞（hPSC）分化获得的心肌细胞则有望为药物筛选、毒性检测及发育研究提供几乎无限的细胞来源。

ScienCell 提供的人多能干细胞来源心肌细胞（HPSC-derived Cardiomyocytes，H9-CM）来源于多能干细胞系（H9），并经过收缩细胞富集。通过对经典 Wnt 信号通路的时序调控及生长因子诱导，在无血清、无饲养层条件下，可将单层培养的 hPSC 高效分化为具有收缩功能的心肌细胞。H9-CM 在富集后于第1代进行冻存，并以冷冻形式交付。每支冻存管含有 >1 × 10⁶ 个细胞，体积为 1 mL。该细胞经免疫荧光鉴定，对 α-辅肌动蛋白（α-Actinin）和肌节原肌球蛋白（Sarcomeric Tropomyosin）抗体呈阳性。H9-CM 经检测不含支原体、细菌、酵母和真菌污染。在 ScienCell 提供的培养条件下，H9-CM 可保证正常培养；但由于其在体外增殖能力有限，不建议用于扩增培养。

推荐培养基

建议在体外培养 H9-CM 时使用心肌细胞培养基（Cardiac Myocyte Medium，CMM，产品编号：6201）。