HIV-1 病毒一步法 RT-PCR 检测试剂盒

HIV-1, human immunodeficiency virus 1, is a type of retrovirus that attacks the human immune system. If HIV-1 infection is not treated with anti-retroviral drugs, it can cause Acquired Immunodeficiency Syndrome, otherwise known as AIDS. There are four groups of HIV-1 virus: M, N, O, and P. The group M is further divided into nine subtypes: A, B, C, D, F, G, H, J, and K. ScienCell's HIV-1 Virus One-step RT-PCR Detection Kit (DHIV1) is designed to detect the presence of HIV-1 virus in cultured cells by one-step reverse transcription PCR (RT-PCR), followed by the electrophoresis analysis of the PCR product. The HIV-1 primer set (included in Cat #7168-ps) targets all four groups, including nine group M subtypes of HIV-1, and gives a positive band at 175 bp. The human control primer set (also included in Cat #7168-ps) targets human B2M gene RNA, but not genomic DNA, with a positive band of 85 bp and acts as a control to assess specimen quality. The positive control (Cat # RU011-pos) consists of noninfectious HIV-1 RNA fragment spiked into human primary cells and serves to ensure reagents and instruments are working properly. In addition, RubyNStart one-step RT-PCR master mix (Cat #MB6058a), RubyNStart enhancer (Cat #MB6058b), cell lysis buffer (Cat #GQ400a), cell lysis buffer enhancer (Cat #GQ400b), and nuclease-free water (Cat #GQ100-4) are included in the kit. Please refer to Tables 4 and 5 for results interpretation.

HIV-1（人类免疫缺陷病毒1型）是一种攻击人体免疫系统的逆转录病毒。如果不使用抗逆转录病毒药物进行治疗，HIV-1 感染可发展为获得性免疫缺陷综合征（AIDS）。HIV-1 病毒分为四个群体：M、N、O 和 P，其中 M 群又进一步分为九个亚型：A、B、C、D、F、G、H、J 和 K。

ScienCell 的 HIV-1 病毒一步法 RT-PCR 检测试剂盒（DHIV1）旨在通过一步法逆转录 PCR（RT-PCR）检测培养细胞中 HIV-1 病毒的存在，并通过对 PCR 产物进行电泳分析来进行结果判定。试剂盒中包含的 HIV-1 引物组（货号 #7168-ps）可检测所有四个群体，包括 M 群的九个亚型，阳性条带为 175 bp。人源对照引物组（同样包含于货号 #7168-ps）靶向人 B2M 基因 RNA（不扩增基因组 DNA），其阳性条带为 85 bp，用作内参以评估样本质量。

阳性对照（货号 #RU011-pos）由掺入人原代细胞中的非感染性 HIV-1 RNA 片段组成，用于确保试剂和仪器正常工作。此外，试剂盒还包含 RubyNStart 一步法 RT-PCR 预混液（货号 #MB6058a）、RubyNStart 增强剂（货号 #MB6058b）、细胞裂解缓冲液（货号 #GQ400a）、细胞裂解缓冲液增强剂（货号 #GQ400b）以及无核酸酶水（货号 #GQ100-4）。结果判读请参见表 4 和表 5。