HiPSC-derived Endothelial Cells
Includes 1 vial of HiPSC-EC (Cat. #1690, 1 mL, >1 x 106 cells/vial), 500 mL Endothelial Cell Medium-basal (ECM, Cat. #1001), 5 mL Endothelial Cell Growth Supplement (ECGS, Cat. #1052), 25mL Fetal Bovine Serum (FBS, Cat. #0025), and 5mL Penicillin-Streptomycin (P/S, Cat. #0503)
Human Endothelial Cells (HiPSC-EC) from ScienCell Research Laboratories are differentiated from a human induced pluripotent stem cell line (HiPSC), which is generated using mRNA reprogramming technology from Human Dermal Fibroblasts (HDF). The monolayer HiPSC efficiently generate mesoderm cells via small-molecule activation of WNT signaling in chemically defined media, and subsequently converted to endothelial cells through endothelial cell specification using vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF).
The derived endothelial cells are characterized by immunofluorescence with antibodies specific to CD31 and vWF. The cell population is highly pure: >80% of cells express CD31 (PECAM-1) & vWF positive. HiPSC-EC are cryopreserved at passage one and delivered frozen. Each vial contains >1 x 106 cells in 1 ml volume. Cells are negative for mycoplasma, bacteria, yeast and fungi. After reviving, HiPSC-EC can be maintained in Endothelial Cell Medium as an adherent culture. HiPSC-EC are not recommended for expanding or long-term cultures.
Recommended Medium
It is recommended to use the provided Endothelial Cell Medium (ECM, Cat. #1001) for plating HiPSC-EC and culturing them.
| 目录编号 | 1690 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | HiPSC-EC |
| 规格/数量 | 1 x 10^6 cells/vial |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后，立即将货号#1690的细胞从干冰直接转移至液氮中保存，直到需要进行实验时再使用。货号#1001存放于4°C，货号#1052、0025和0503存放于-20°C。 |
| 运输 | Cat. #1690、1052、0025 和 0503 使用干冰运输。Cat. #1001 常温运输。 |
