Glucose-6-phosphate Dehydrogenase Assay

Glucose-6-phosphate dehydrogenase (G6PDH) is a cytosolic enzyme that catalyzes the first step in the pentose phosphate pathway (PPP). The PPP pathway is critical for maintaining the cofactor nicotinamide adenine dinucleotide phosphate (NADPH) and for the production of ribose-5-phosphate. G6PDH deficiency, the most common enzyme deficiency worldwide, predisposes individuals to non-immune hemolytic anemia. This colorimetric assay is based on glucose-6-phosphate dehydrogenase catalyzed oxidation of glucose-6-phosphate, in which the formed NADPH can convert a nearly colorless probe to an intensely colored product, which exhibits maximum absorbance at 440nm, is proportional to the amount of G6PDH in the sample.