GeneQuery™ Mouse cDNA Evaluation Kit, 100 reactions
Each primer set included in GQM-CE qPCR kit arrives lyophilized in a 2 mL vial. All primers are designed and tested under the same parameters.
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This Item:
US$109.00
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ScienCell's GeneQuery™ Mouse cDNA Evaluation Kit (GQM-CE) assesses mouse cDNA quality. The kit verifies successful reverse transcription of messenger RNA (mRNA) to complementary DNA (cDNA) and reveals the presence of mouse genomic DNA (gDNA) contamination in mouse cDNA samples. Good quality cDNA is a critical component for successful gene expression profiling. The GQM-CE kit is highly recommended for cDNA applications such as GeneQuery™ qPCR arrays.
Each primer set included in GQM-CE qPCR kit arrives lyophilized in a 2 mL vial. All primers are designed and tested under the same parameters: (i) an optimal annealing temperature of 65°C (with 2 mM Mg2+, and no DMSO); (ii) recognition of all known target gene transcript variants; and (iii) specific amplification of only one amplicon. Each primer set has been validated by qPCR by melt curve analysis and gel electrophoresis.
Quality Control
Each primer set is validated by qPCR melt curve and amplification curve analyses. The PCR products are analyzed by gel electrophoresis to confirm single band amplification.
| 目录编号 | GK992M |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | GQM-CE |
| 规格/数量 | One 96-well plate, one 20ul reaction per well |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 该产品常温运输。收到后，小瓶应储存在 4°C，可保存长达 12 个月。长期储存（超过 1 年）请将小瓶储存在手动除霜的 -20°C 冰箱中。 |
| 运输 | 常温。 |
