GeneQuery™ Human TLR Signaling Pathway qPCR Array Kit

ScienCell's GeneQuery™ Human Toll-like receptor (TLR) Signaling Pathway qPCR array kit profiles 88 key genes associated with the TLR Signaling Pathway. The TLR pathway is a key part of the natural immune system and serves as an integral defense mechanism against invaders. Through the recognition of pathogen-associated molecular patterns (PAMPs), such as bacterial lipopolysaccharides and viral nucleic acids, TLRs trigger a cascade of signaling events that result in the activation of various transcription factors, interferons, chemokines, and the production of pro-inflammatory cytokines. ScienCell's GeneQuery™ Human TLR Signaling Pathway qPCR Array Kit offers a comprehensive platform for investigating the expression profiles of key genes involved in TLR signaling. Researchers can gain valuable insights into both the physiological roles of TLRs in host defense and their contributions to immune-related disorders. Brief examples of how included genes may be grouped according to function are shown below:

• Receptor: TLR1, TLR2, TLR3, TLR4, TLR5, TLR6, TLR7, TLR8, TLR9, TLR10
• Ligand: CCL2, CCL4, CCL5, CXCL10, CXCL11, CXCL8, CXCL9
• Kinase: MAPK1, MAPK14, MAPK8, MAPK9, MAP2K2, MAP2K3, MAP2K4
• Cytokine: IFNA1, IFNB1, IFNG, IL1A, IL1B, TNF

GeneQuery™ qPCR array kits are qPCR-ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.