GeneQuery™ Human JAK-STAT Signaling Pathway qPCR Array Kit

ScienCell's GeneQuery™ Human Janus Kinase/Signal Transducers and Activators of Transcription (JAK-STAT) Signaling Pathway qPCR array Kit profiles 88 key genes associated with the JAK-STAT Signaling Pathway. This pathway regulates essential cellular processes such as growth, differentiation, immune response, and inflammation. Activation of the JAK-STAT signaling typically occurs in response to cytokines and growth factors binding to their respective cell surface receptors, triggering a cascade of phosphorylation events that ultimately lead to the activation of transcription factors and subsequent gene expression changes. Dysregulation of the JAK-STAT pathway has been implicated in numerous diseases, including cancer, autoimmune disorders, and inflammatory conditions. ScienCell's GeneQuery™ Human JAK-STAT Signaling Pathway qPCR Array Kit offers a comprehensive tool for examining the expression profiles of key genes involved in this pathway, facilitating the study of its regulation and potential therapeutic targets. Brief examples of how included genes may be grouped according to function are shown below:

• Receptor: FAS, GRB2, GHR, IFNAR1, IFNGR1, IFNGR2, IL4R
• DNA-binding: ATF3, IRF1, NFKB1, STAT1, STAT2, STAT3
• Kinase: AKT1, AKT2, JAK1, JAK2. JAK3, AURKB, EIF2AK3, MAPK3
• Cytokine: CXCL9, IFNG, IL10, IL11, IL4, IL6, THPO

GeneQuery™ qPCR array kits are qPCR-ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.