GeneQuery™ 人焦点黏附复合体 qPCR 阵列试剂盒

ScienCell's GeneQuery™ Human Focal Adhesion Complexes qPCR Array Kit (GQH-FAC) profiles 88 key genes involved in the regulation and formation of focal adhesions. Focal adhesions are large macromolecular complexes by which the cell interacts with its extracellular matrix (ECM) environment. They are involved in such diverse processes as interpreting mechanical force, regulating cell adhesion and motility, and contributing to intracellular signaling transduction. Integrins and focal adhesion kinase (PTK2) are the main regulatory elements of focal adhesions. Brief examples of how included genes may be categorized are shown below:

• FAK signaling: CCND1, FYN, PTK2, RHOA, SRC
• Cytoskeletal elements: ACTB, ACTN1, ACTN3, FLNA, VCL
• ECM components: COL1A2, COL2A1, COL4A2, FN1, LAMC3
• ECM binding elements: ITGA1, ITGA5, ITGAM, ITGB1, ITGB3
• Signaling transduction genes: ARHGAP5, ELK1, HGF, JUN, KDR

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺ and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

ScienCell 的 GeneQuery™ 人焦点黏附复合体 qPCR 阵列试剂盒（GQH-FAC）可检测参与焦点黏附形成与调控的 88 个关键基因的表达。焦点黏附是细胞与其周围细胞外基质（ECM）相互作用的大型高分子复合体，参与机械力感知、细胞黏附与迁移调控以及细胞内信号转导等多种重要生物学过程。整合素（Integrins）和黏着斑激酶（Focal Adhesion Kinase，FAK；PTK2）是焦点黏附的重要调控因子。试剂盒所包含的部分基因可按功能分类如下：

• FAK 信号通路：CCND1、FYN、PTK2、RHOA、SRC
• 细胞骨架相关基因：ACTB、ACTN1、ACTN3、FLNA、VCL
• 细胞外基质（ECM）组成成分：COL1A2、COL2A1、COL4A2、FN1、LAMC3
• ECM 结合相关分子：ITGA1、ITGA5、ITGAM、ITGB1、ITGB3
• 信号转导相关基因：ARHGAP5、ELK1、HGF、JUN、KDR

GeneQuery™ qPCR 阵列试剂盒采用预制的 96 孔板形式，可直接用于 qPCR 检测。每个反应孔均预装一组特异性引物，用于识别并高效扩增对应目标基因的 cDNA。经过精心优化设计的引物可确保：（i）在 qPCR 分析条件下（2 mM Mg²⁺、不添加 DMSO），最佳退火温度为 65°C；（ii）除非另有说明，每组引物均可识别目标基因所有已知的转录本变体；（iii）每组引物仅特异性扩增对应的目标基因。所有引物均经过 qPCR 熔解曲线分析和凝胶电泳验证，以确保其扩增特异性和可靠性。

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