GeneQuery™ Human Diabetes, Type I (Juvenile, Insulin-dependent) qPCR Array Kit

ScienCell's GeneQuery™ Human Diabetes, Type I (Juvenile, Insulin-dependent) qPCR Array Kit (GQH-DB1) is designed to facilitate gene expression profiling of 88 key genes involved in the development and progression of insulin-dependent diabetes mellitus. Type 1 diabetes mellitus is characterized by abnormally high blood sugar levels resultant of a disorder in glucose homeostasis. Brief examples of how included genes may be grouped according to function are shown below:

• Blood glucose regulation: INS, INSR, SLC2A2, SLC30A8, ABCC8
• Autoimmunity: GAD1, GAD2, LTA, HLA-A, OAS1
• Fat metabolism: ADIPOQ, LPL, PON1, LEP, RETN
• Cholesterol regulation: APOA1, APOB, LPA, CETP, AMBP
• Hormone regulation: PREP, IGF1, SST, HGF, REN

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.