GeneQuery™ HLA-DQB1 PCR+Sanger SBT Typing Kit

Name: GeneQuery™ HLA-DQB1 PCR+Sanger SBT Typing Kit
Brand: Sciencell Research Laboratories
SKU: GK895
Price: 682 USD
Availability: InStock

Catalog No.

GK895

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GeneQuery™ HLA-DQB1 PCR+Sanger SBT Typing Kit (SBT-DQB1) utilizes the Sequence-based Typing (SBT) method, which is the gold standard for HLA typing. This method involves PCR amplification of HLA genes, followed by Sanger sequencing of the amplicons. The acquired sequences are aligned to an HLA allele database using the SOAPTyping software (BGI Genomics) to obtain the 4-digit genotypes of the samples. The two haplotypes from both parents can be typed simultaneously using this kit and the SOAPTyping software.

Kit Components:

Cat #	Component	Quantity	Storage
GK895a	HLA-DQB1 amplification primer set (Q_ampF and Q_ampR)	400 µL	-20°C
GK895b	Q_2F sequencing primer mix, 10 µM	200 μL	-20°C
GK895c	Q_2R sequencing primer mix, 10 µM	200 μL	-20°C
GK895d	Q_3F sequencing primer mix, 10 µM	200 μL	-20°C
GK895e	Q_3R sequencing primer mix, 10 µM	200 μL	-20°C
GK895h	Nuclease-free H₂O	4 mL	4°C
GK895r	Human genomic DNA reference (GDR), ~50 ng/µL (HLA-DQB102:01 and HLA-DQB106:02)	10 μL	-20°C
MB6038-1	2X EmeraldNStart HiFi Marathon PCR Master Mix, 1 mL	2 vials	-20°C