GeneQuery™ Human Mesenchymal Stem Cell Adipogenesis qPCR Array Kit

ScienCell's GeneQuery™ Human Mesenchymal Stem Cell Adipogenesis qPCR Array Kit (GQH-MAD) is designed to facilitate gene expression profiling of 88 key genes involved in mesenchymal stem cell (MSC) adipogenic lineage commitment and differentiation. MSCs have the capacity to self-renew while maintaining stemness, and have the potential to differentiate into specialized cell lineages including adipocytes. The PPAR-gamma signaling pathway plays a key role in the induction and regulation of the adipogenesis process. Brief examples of how genes may be grouped are shown below:

• MSC markers: ALCAM, ANPEP, CASP3, CD44, ENG, ERBB2, ITGA1/6/V, MCAM, NGFR, NT5E, PDGFRA/B, THY1, VCAM1, VIM
• Stemness markers: FGF2, FUT4, INS, LIF, POU5F1, PROM1, SOX2, TERT, WNT3A, ZFP42
• Adipogenic commitment regulation: ADIPOR1/2, AXIN1, CCND1, CEBPB/D, CIDEA, DLK1/2, EGR2, FGF1, FOXO1, GATA2/3, KLFs, LEP, LEPR, RHOA, SLC27A1, TNFRSF9, VDR
• PPAR-gamma signaling: ADIPOQ, AGT, CEBPA, CREB1, FABP4, IRS2, KLF15, PPARG, RETN, SIRT3, SREBF1, STAT5A
• Fully differentiated adipocyte markers: FASN, LIPE, LPL, PPARGC1A, SLC2A4, UCP1

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.