GeneQuery™ 人脑发育标志物 qPCR 检测芯片试剂盒

ScienCell's GeneQuery™ Human Brain Development Markers qPCR Array Kit (GQH-BDM) surveys a panel of 24 marker genes involved in brain development, function and pathogenesis. GQH-BDM is also useful for evaluating the roles of stem and progenitor cells in brain organoid generation. Brief examples of how genes may be grouped are shown below:

• M1 microglia phenotype markers: TNF, NOS2, IL1B
• M2 microglia phenotype markers: IL10, ARG1, MRC1
• Pericyte markers: ACTA2, PDGFRB, CSPG4
• Evaluation of neural differentiation
  • Astrocyte markers:   GFAP, S100B, GLUL
  • Immature neuron markers:   NEUROD1, DCX, TUBB3
  • Mature neuron markers:   MAP2, RBFOX3, SYP
• Neuron function evaluation: CHAT, TH (tyrosine hydroxylase), MAPT
• Blood-brain barrier (BBB) evaluation: VCAM1, OCLN, ICAM1

Note: all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg ²⁺, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

ScienCell 的 GeneQuery™ 人脑发育标志物 qPCR 检测芯片试剂盒（GQH-BDM）涵盖 24 种参与脑发育、脑功能调节及疾病发生机制的标志基因，可用于分析相关基因的表达情况。此外，该试剂盒还可用于评估干细胞和祖细胞在脑类器官构建中的作用。以下列举了部分基因的功能分类示例：

• M1 型小胶质细胞表型标志物： TNF、NOS2、IL1B
• M2 型小胶质细胞表型标志物： IL10、ARG1、MRC1
• 周细胞标志物： ACTA2、PDGFRB、CSPG4
• 神经细胞分化评估：
  • 星形胶质细胞标志物： GFAP、S100B、GLUL
  • 未成熟神经元标志物： NEUROD1、DCX、TUBB3
  • 成熟神经元标志物： MAP2、RBFOX3、SYP
• 神经元功能评估： CHAT、TH（酪氨酸羟化酶）、MAPT
• 血脑屏障（BBB）功能评估： VCAM1、OCLN、ICAM1

注意：所有基因名称均采用人类基因组命名委员会（HGNC）制定的官方基因符号。

GeneQuery™ qPCR 检测芯片试剂盒采用 96 孔板形式，可直接用于 qPCR 实验。每个孔中均预置一组引物，可特异性识别并高效扩增目标基因的互补 DNA（cDNA）。引物经过精心设计，以确保以下性能：（1）在 qPCR 分析中，最佳退火温度为 65°C（Mg²⁺ 浓度为 2 mM，且不添加 DMSO）；（2）除非另有说明，每组引物均可识别目标基因所有已知的转录本变体；（3）每组引物仅扩增一个目标基因。所有引物组均经过 qPCR 熔解曲线分析和凝胶电泳验证。

Search for a Certificate of Analysis by Lot Number below:

Frequently Asked Questions →

Find answers to common questions about ScienCell products, cell culture, handling, and research applications.

No Publication available at this time.