G4 EA H1N1 猪流感病毒 RT-qPCR 检测试剂盒

Pigs have been shown to be key hosts for the generation of pandemic influenza viruses including the one that caused the 2009 H1N1 pandemic. Since 2016, a recently identified H1N1 swine flu virus genotype, the genotype 4 reassortant Eurasian avian-like (G4 EA) H1N1 virus, has rapidly spread and become the predominant genotype in the surveilled pig populations in China. The G4 EA H1N1 swine flu virus also demonstrated human infectivity and has the potential to become a pandemic virus.

ScienCell's G4 EA H1N1 Swine Flu Virus RT-qPCR Detection Kit (G4EASF) is designed to detect the presence of influenza A viruses and the newly emerged genotype G4 EA H1N1 Swine Flu virus in extracted RNA samples. Three primer/probe sets are included in the kit. Two of them (Cat #7068-InfA and 7068-G4EA) target a universal region shared by all influenza A viruses and a region specific to G4 EA H1N1 virus, respectively. The human ACTB gene primer/probe set (Cat #7068-ACTB) targets the human β-actin (ACTB) housekeeping gene, which serves as an internal control to assess specimen quality. All primer/probe sets are verified to possess high specificity and efficiency near 100% under recommended PCR conditions. In addition, a non-infectious positive control (Cat #7068-Pos) and nuclease-free water are included in the kit. The positive control (Cat #7068-Pos) consists of non-infectious viral RNA fragments and serves to ensure reagents and instruments are working properly. Please refer to Tables 4 and 5 for results interpretation.

研究表明，猪是产生大流行性流感病毒的重要宿主之一，包括引发2009年 H1N1 大流行的病毒。自2016年以来，一种新近鉴定的 H1N1 猪流感病毒基因型——4型重配欧亚类禽源（G4 EA）H1N1 病毒，已迅速传播，并成为中国监测猪群中的主要流行基因型。G4 EA H1N1 猪流感病毒也已表现出对人类的感染能力，并具有成为大流行病毒的潜力。

ScienCell 的 G4 EA H1N1 猪流感病毒 RT-qPCR 检测试剂盒（G4EASF）旨在检测提取的 RNA 样本中是否存在甲型流感病毒以及新出现的 G4 EA H1N1 猪流感病毒基因型。试剂盒中包含三套引物/探针。其中两套（货号 #7068-InfA 和 7068-G4EA）分别靶向所有甲型流感病毒共有的通用区域以及 G4 EA H1N1 病毒特异区域。人 ACTB 基因引物/探针（货号 #7068-ACTB）用于检测人 β-肌动蛋白（ACTB）管家基因，作为内参对照以评估样本质量。所有引物/探针组合在推荐的 PCR 条件下均经验证具有高特异性和接近 100% 的扩增效率。此外，试剂盒中还包含非感染性阳性对照（货号 #7068-Pos）和无核酸酶水。阳性对照（货号 #7068-Pos）由非感染性病毒 RNA 片段组成，用于确保试剂和仪器运行正常。结果判读请参见表 4 和表 5。