DMEM 培养基（含 L-谷氨酰胺、丙酮酸钠和 HEPES）

Many modifications of eagle's medium have been developed since the original formulation. The most widely used modification is Dulbecco's Modified Eagle's Medium (DMEM), which was developed in 1969. HEPES buffer is included in DMEM formulation at a final concentration of 25 mM to compensate for the loss of buffering capacity incurred by eliminating serum. DMEM with L-Glutamine, Sodium Pyruvate and HEPES (09231) is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals. DMEM is well suited for supporting the growth of a broad range of mammalian cell lines.

DMEM with L-Glutamine, Sodium Pyruvate and HEPES is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

自 Eagle 培养基最初配方问世以来，研究人员已开发出多种改良配方，其中应用最广泛的是 1969 年推出的 Dulbecco 改良 Eagle 培养基（Dulbecco's Modified Eagle's Medium，DMEM）。为弥补去除血清后缓冲能力的降低，DMEM 配方中添加了终浓度为 25 mM 的 HEPES 缓冲剂，以增强培养基的 pH 稳定性。

DMEM 培养基（含 L-谷氨酰胺、丙酮酸钠和 HEPES）（09231）为无菌液体培养基，含有必需氨基酸和非必需氨基酸、维生素、有机和无机化合物、激素、生长因子以及微量矿物质等多种营养成分，可广泛用于支持多种哺乳动物细胞系的生长。

DMEM 培养基（含 L-谷氨酰胺、丙酮酸钠和 HEPES）仅供科研使用，不得用于人体、动物或体外诊断用途。