Colorimetric TUNEL Apoptosis Assay

The ScienCell Colorimetric TUNEL Apoptosis Assay is used for detection of apoptosis (programmed cell death) in individual cells based on the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick-end-labeling (TUNEL) technology. In brief, the 3'-OH end of the DNA strand breaks in apoptotic cells is labeled with biotinylated nucleotides using the enzyme TdT. Streptavidin conjugated horseradish peroxidase (HRP) is then bound to the biotinylated nucleotides, and visualized using the peroxidase substrate, 3,3'-diaminobenzidine (DAB). The nuclei of apoptotic cells should be observed dark brown under light microscope.