GAPDH 比色法检测

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) is a tetrameric enzyme that catalyzes glycolysis and thus serves to break down glucose for energy and carbon molecules. Since it is ubiquitously expressed at relatively constant levels, GAPDH gene is widely used as an internal control for RNA and protein analysis. The ScienCell™ Colorimetric GAPDH Assay provides a non-radioactive method to measure the enzyme activity of GAPDH in cultured cells, based on the oxidization of β-NADH to β-NAD in the presence of 3-phosphoglyceric acid (3-PGA), adenosine 5'-triphosphate (ATP) and GAPDH. The GAPDH activity is determined by assaying the rate of NADH oxidation, which is proportional to the reduction in absorbance at 340 nm over time (ΔA_340nm/min).

甘油醛-3-磷酸脱氢酶（Glyceraldehyde-3-Phosphate Dehydrogenase，GAPDH）是一种四聚体酶，可催化糖酵解过程，在葡萄糖分解以产生能量和碳源分子中发挥重要作用。由于 GAPDH 在多种组织和细胞中广泛表达，且表达水平相对稳定，因此其基因常被用作 RNA 和蛋白质分析的内参基因。

ScienCell™ GAPDH 比色法检测试剂盒采用非放射性方法检测培养细胞中 GAPDH 的酶活性。其检测原理是在 3-磷酸甘油酸（3-PGA）、三磷酸腺苷（ATP）和 GAPDH 存在的条件下，催化 β-NADH 氧化生成 β-NAD。通过测定 NADH 氧化速率，即监测 340 nm 波长处吸光度随时间的降低速率（ΔA_340nm/min），即可定量分析 GAPDH 的酶活性。

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