Collagen I Cell Adhesion Assay

Cell adhesion plays an important role in cellular communication and regulation, and is of fundamental importance in the development and maintenance of tissues. Collagen I, a major structural component of extracellular matrices, is an excellent substrate for the culture of many different cell types. The ScienCell' Collagen I Cell Adhesion Assay is designed for the rapid, quantitative and reliable measurement of cell adhesion to collagen I. The kit includes a 48-well plate pre-coated with collagen I, as well as BSA (bovine serum albumin) as negative controls (see plate format in Figure 1). Cells are cultured in the pre-coated wells for a desired period of time, then unbounded cells are washed away, and the adhered cells are fixed and stained, followed by an extraction step which leads to dye elution from stained cells into supernatant. Thus cell adhesion can be quantified using a colorimetric ELISA plate reader at 595 nm.