I型胶原细胞黏附实验

Cell adhesion plays an important role in cellular communication and regulation, and is of fundamental importance in the development and maintenance of tissues. Collagen I, a major structural component of extracellular matrices, is an excellent substrate for the culture of many different cell types. The ScienCell' Collagen I Cell Adhesion Assay is designed for the rapid, quantitative and reliable measurement of cell adhesion to collagen I. The kit includes a 48-well plate pre-coated with collagen I, as well as BSA (bovine serum albumin) as negative controls (see plate format in Figure 1). Cells are cultured in the pre-coated wells for a desired period of time, then unbounded cells are washed away, and the adhered cells are fixed and stained, followed by an extraction step which leads to dye elution from stained cells into supernatant. Thus cell adhesion can be quantified using a colorimetric ELISA plate reader at 595 nm.

细胞黏附在细胞间信息传递和功能调控中发挥着重要作用，是组织发育和维持正常生理功能的重要基础。I 型胶原是细胞外基质（ECM）的主要结构成分之一，可为多种细胞的培养提供理想的黏附基质。ScienCell 的 I 型胶原细胞黏附检测试剂盒可快速、定量且可靠地检测细胞对 I 型胶原的黏附能力。

本试剂盒提供一块预包被 I 型胶原的 48 孔板，并配有以 BSA（牛血清白蛋白）包被的阴性对照孔（孔板布局见图 1）。实验时，将细胞接种至预包被孔中培养至所需时间，随后洗去未黏附的细胞，对黏附细胞进行固定和染色，再通过染料提取步骤将染色细胞中的染料释放至上清液中。最后，使用酶联免疫吸附测定（ELISA）酶标仪在 595 nm 波长下进行比色检测，即可对细胞黏附水平进行定量分析。