Citrate Synthase Assay
This kit is used for the fast and simple measurement of citrate synthase activity as well as the detection of intact mitochondrial inner membrane. It is for research purposes only and not for use in animals, humans, or diagnostic procedures.
Citrate synthase is the initial enzyme of the tricarboxylic acid (TCA) cycle. This enzyme catalyzes the reaction between acetyl coenzyme A (acetyl CoA) and oxaloacetic acid to form citric acid and CoA with a thiol group (CoA-SH). This colorimetric assay is based on the reaction between 5, 5’-Dithiobis 2nitrobenzoic acid (DTNB) and CoA-SH to form TNB, which exhibits maximum absorbance at 412 nm. The intensity of the absorbance is proportional to the citrate synthase activity. This enzyme is an exclusive marker of the mitochondrial matrix1.
| 目录编号 | 8318 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | CS |
| 规格/数量 | 100 tests in 96-well plate |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 请参阅产品说明书。 |
| 运输 | 干冰。 |