牛血浆玻连蛋白
ScienCell™ 牛血浆玻连蛋白(Bovine Plasma Vitronectin)通过亲和层析法从牛血浆中纯化获得。本产品以 Dulbecco 磷酸盐缓冲盐水(DPBS)为溶液体系,无菌供应。 注意:如果操作不当,本产品中的某些成分可能对健康造成危害。使用时请采取适当的防护措施,包括佩戴防护服和防护眼镜,并按照相关规定进行妥善处理。
Vitronectin is a glycoprotein found in plasma and extracellular matrix (ECM). It is a soluble, disulfide-linked dimer, composed of a 75 kDa and a 65 kDa peptide chain. In plasma, vitronectin is predominantly found as a single chain monomer [1]. The N-terminal of vitronectin contains multiple binding sites for a variety of structures. Vitronectin is involved in a number of biological functions including cell adhesion, spreading, migration, proliferation, extracellular anchoring, fibrinolysis, hemostasis, and complement immune defense [2, 3, 4]. Vitronectin can be used for coating tissue culture surfaces to promote cell adhesion, proliferation, and differentiation.
ScienCell™ Bovine Plasma Vitronectin is purified from bovine plasma by affinity chromatography [5]. It is supplied as a sterile solution in Dulbecco's Phosphate-Buffered Saline (DPBS). Optimal conditions for cell attachment must be determined for each cell line and application.
Product Specification
Quantity: 100 µg
Concentration: 0.4 mg/ml
Storage buffer: DPBS, pH 7.4
玻连蛋白(Vitronectin)是一种存在于血浆和细胞外基质(ECM)中的糖蛋白。它是一种可溶性、通过二硫键连接的二聚体,由 75 kDa 和 65 kDa 两条多肽链组成。在血浆中,玻连蛋白主要以单链单体形式存在 [1]。
玻连蛋白的 N 端 含有多个可与多种分子结构结合的位点。它参与多种重要的生物学过程,包括细胞黏附、细胞铺展、细胞迁移、细胞增殖、细胞外基质锚定、纤溶作用、止血过程以及补体介导的免疫防御等 [2,3,4]。在细胞培养中,玻连蛋白常用于包被培养表面,以促进细胞黏附、增殖和分化。
ScienCell™ 牛血浆玻连蛋白(Bovine Plasma Vitronectin)通过亲和层析法从牛血浆中纯化获得 [5],并以Dulbecco 磷酸盐缓冲盐水(DPBS)形式提供,为无菌溶液。不同细胞系及应用条件下的最佳细胞贴壁条件需通过实验确定。
产品规格(Product Specification)
规格(Quantity): 100 μg
浓度(Concentration): 0.4 mg/ml
储存缓冲液(Storage buffer): DPBS,pH 7.4
| 目录编号 | 8538 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | BPV |
| 规格/数量 | 100 ug |
| 产品用途 | 本产品仅供研究使用。未经批准,不得用于人体、动物或体外诊断程序。 |
| 储存 | 建议将产品分装并存放于 -80°C。Vitronectin 应在 2-8°C 下缓慢解冻,避免摇晃。如有沉淀可通过离心去除。避免反复冻融。 |
| 运输 | 干冰。 |