牛源脑微血管内皮细胞

Brain microvascular endothelial cells (BMEC), the major component of the blood-brain barrier, limit the passage of substances, both soluble and cellular, from the blood into the brain. BMEC utilize unique features to distinguish themselves from peripheral endothelial cells, such as 1) intercellular tight junctions that display high electrical resistance and slow paracellular transport, 2) the absence of fenestrae and a reduced level of pinocytic activity, and 3) the expression of specialized pumps that can transport compounds out of the brain via the blood-brain barrier. Similar to peripheral endothelial cells, BMEC express, or can be induced to express, cell adhesion molecules on their surface that regulate the extravasation of leukocytes into the brain. Cultured bovine BMEC have been used for studying the molecular and cellular properties of blood-brain barrier because of their unique functions. Understanding the molecular mechanisms of blood-brain barrier regulation may help to optimize drug delivery to the CNS and elucidate new therapies for CNS diseases.

BBMEC from ScienCell Research Laboratories are isolated from bovine brain. BBMEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. BBMEC are characterized by immunofluorescence with antibody specific to vWF/Factor VIII. BBMEC are negative for mycoplasma, bacteria, yeast, and fungi. BBMEC are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories. 

Recommended Medium
It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for culturing BBMEC in vitro.

脑微血管内皮细胞（BMEC）是血脑屏障（BBB）的主要组成部分，可限制血液中可溶性物质及细胞成分向脑组织的跨越，从而维持中枢神经系统的稳态。BMEC具有区别于外周内皮细胞的独特结构与功能特征，包括：1）细胞间紧密连接结构完善，具有高电阻特性并显著降低细胞旁路转运；2）缺乏窗孔结构且胞饮作用水平较低；3）表达多种特异性外排转运蛋白，可将物质从脑组织泵出至血液循环。此外，与外周内皮细胞类似，BMEC可表达或诱导表达细胞黏附分子，参与调控白细胞向脑组织的跨内皮迁移。

由于其独特的生理功能，培养的牛源BMEC常用于血脑屏障分子与细胞机制研究，有助于深入理解BBB调控机制，从而优化中枢神经系统药物递送策略并探索神经系统疾病的新型治疗方法。

ScienCell研究实验室提供的BBMEC来源于牛脑组织分离。细胞在P1代进行冻存并以冻存状态运输，每支冻存管含有 >5 × 10⁵ 个细胞（1 mL）。BBMEC通过针对vWF/Factor VIII的免疫荧光染色进行鉴定，并确认无支原体、细菌、酵母及真菌污染。产品保证在ScienCell提供的培养条件下可至少传代扩增5个群体倍增（population doublings）。

推荐培养基：建议使用内皮细胞培养基（Endothelial Cell Medium，ECM，产品编号 1001）进行体外培养。