bFGF-Stimulated Mouse Embryonic Fibroblast Conditioned Medium

bFGF-Stimulated Mouse Embryonic Fibroblast-Conditioned Medium (bFGF-std MEF-cm) is a complete serum-free medium for the feeder-free culture of human pluripotent stem cells (hPSCs), including embryonic stem cells and induced pluripotent stem cells. bFGF-std MEF-cm is a complete medium containing recombinant human basic FGF (rhbFGF) and is ready to use after thawing, so there is no need to add extra basic FGF. It supports the self-renewal of hPSC and maintains pluripotency in the presence of hPSC qualified matrix (e.g., BD MatrigelTM matrix). bFGF-std MEF-cm was prepared using high quality mitomycin C treated MEF (MEF-mt, Cat. #M7570-mt) derived from E13 embryos of CF1 mice. Serum free medium was conditioned with mitomycin C treated CF1 MEFs for 24 hours. The conditioned medium was collected daily for 7 days, pooled and sterilized by filtering through a 0.22 μm filter. Recombinant human basic FGF (rhbFGF, Cat. #104-02) was added to the medium at a final concentration of 4 ng/mL to make the complete medium. bFGF-std MEF-cm has been tested for its ability to support hPSC self-renewal on BD MatrigelTM matrix in vitro. The medium color of each lot may vary but does not affect performance.