大鼠线粒体DNA拷贝数绝对定量 qPCR 检测试剂盒

Mitochondrial DNA (mtDNA) is circular, multicopy genome DNA located in mitochondrion, a cellular organelle that plays a key role in energy production of the cell. The capacity for energy production in a cell depends on both mtDNA integrity and copy number. Substantial evidence suggests that alterations in mtDNA copy number have been correlated with aging and various age-related disorders, such as cancer, diabetes and neurodegenerative diseases.

ScienCell's Absolute Rat Mitochondrial DNA Copy Number Quantification qPCR Assay Kit (ARMQ) is designed to directly compare the average mtDNA copy number of the samples. The rat mtDNA primer set recognizes and amplifies one of the most conserved regions on rat mtDNA and will not amplify any off-target sequence on nuclear genomic DNA. The single copy reference (SCR) primer set recognizes and amplifies a 100 bp-long region on rat chromosome 17 and serves as reference for data normalization. The reference genomic DNA sample with known mtDNA copy number serves as a reference for calculating the mtDNA copy number of target samples. The carefully designed primers ensure: (i) high efficiency for trustworthy quantification; and (ii) no non-specific amplification. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis for amplification specificity and by template serial dilution for amplification efficiency.

线粒体DNA（mtDNA）是一种位于线粒体中的环状、多拷贝基因组DNA。线粒体作为细胞内的重要细胞器，在细胞能量产生过程中发挥关键作用。细胞的能量生成能力取决于mtDNA的完整性及其拷贝数。大量研究表明，mtDNA拷贝数的变化与衰老以及多种与年龄相关的疾病密切相关，例如癌症、糖尿病和神经退行性疾病。

ScienCell 大鼠线粒体DNA拷贝数绝对定量 qPCR 检测试剂盒（ARMQ）用于直接比较不同样品中mtDNA的平均拷贝数。该试剂盒中的大鼠mtDNA引物组可识别并扩增大鼠mtDNA上高度保守的区域，且不会扩增核基因组DNA中的非特异性序列。单拷贝参考基因（SCR）引物组针对大鼠第17号染色体上一段约100 bp的区域进行扩增，作为数据标准化的内参。具有已知mtDNA拷贝数的参考基因组DNA样本可作为标准，用于计算目标样品中的mtDNA拷贝数。

精心设计的引物可确保：（i）高扩增效率，从而获得可靠的定量结果；（ii）无非特异性扩增。每组引物均通过qPCR熔解曲线分析和凝胶电泳验证其扩增特异性，并通过模板梯度稀释实验验证其扩增效率。