3D肾小管形成试剂盒

The human kidney is frequently exposed to drugs and toxic compounds, which can result in nephrotoxicity. A drug's uptake and clearance from the kidney largely relies on a subset of membrane transporters such as organic anion transporters (OAT) 1/3, organic cation transporter (OCT) 2, and multidrug and toxin extrusion proteins (MATE) 1/2. In 2D monolayer culture, primary renal epithelial cells rapidly lose the expression of OAT1/3, OCT2, and MATE1/2, limiting the accurate redictability of a drug's effect [1]. ScienCell has developed a 3D renal tubule formation kit (3D-RTF) using human primary renal cortical epithelial cells isolated from normal adult kidney tissue, which are then embedded in Collagen I extracellular matrix. We demonstrated that renal tubule structures are formed in a robust and reproducible manner in a 3D collagenembedded model using the 3D-RTF. 3D cultured renal epithelial cells had elevated expression levels of xenobiotic transporters such as OAT1/3, and MATE1/2 (see Figure 1 and 2). The 3DRTF provides a useful in vitro tool for accurately predicting a drug's effect on kidney function.

人肾脏经常暴露于药物和有毒化合物，因此容易发生肾毒性。药物在肾脏中的摄取和清除主要依赖于一类膜转运蛋白，包括有机阴离子转运蛋白（OAT）1/3、有机阳离子转运蛋白（OCT）2以及多药和毒素外排蛋白（MATE）1/2。在二维（2D）单层培养条件下，原代肾上皮细胞会迅速丧失OAT1/3、OCT2和MATE1/2的表达，从而限制了对药物作用的准确预测能力[1]。 ScienCell利用分离自正常成人肾组织的人原代肾皮质上皮细胞，并将其包埋于Ⅰ型胶原细胞外基质中，开发了3D肾小管形成试剂盒（3D-RTF）。研究表明，在该基于三维胶原基质的模型中，可稳定且可重复地形成肾小管样结构。与二维培养相比，三维培养的肾上皮细胞表现出更高水平的异生物转运蛋白表达，包括OAT1/3和MATE1/2（见图1和图2）。 3D-RTF为准确预测药物对肾功能的影响提供了一种具有高度生理相关性的体外研究工具。