3D-Preadipocyte Spheroid Differentiation Medium

3D-PADM consists of 200 mL of basal medium, 2 mL of 3D-preadipocyte spheroid differentiation supplement (3D-PAdDS, Cat. #3D-7232), 10 mL of fetal bovine serum (FBS, Cat. #0010) and 2 mL penicillin/streptomycin solution (P/S, Cat. #0583). Note: 3D-PAdDS, FBS and P/S are not pre-mixed in the 3D-PADM; they must be added separately to make the complete medium.