All-Inclusive 3D Human Hepatic Stellate-Endothelial Cell Spheroid Formation Kit
ScienCell has developed the all-inclusive 3D human hepatic stellate-endothelial cell spheroid formation kit (3D-HHSteECSF) comprised of human hepatic stellate cells and sinusoidal endothelial cells. This kit is designed for studying the cellular crosstalk between hepatic stellate cells and sinusoidal endothelial cells during liver diseases such as liver fibrosis and caner metastasis.
Liver fibrogenesis and angiogenesis are closely linked processes. For example, in physiological conditions, hepatic sinusoidal endothelial cells (HSEC) maintain hepatic stellate cell (HSteC) quiescence, thereby inhibiting intrahepatic vasoconstriction and fibrosis development. When capillarized, however, hepatic sinusoidal endothelial cells lose their capacity to inactivate hepatic stellate cells, thus promoting fibrogenesis and intrahepatic vasoconstriction. To investigate the signaling crosstalk between these cellular events, ScienCell has developed the all-inclusive 3D human hepatic stellate-endothelial cell spheroid formation kit (3D-HHSteECSF) comprised of human hepatic stellate cells and sinusoidal endothelial cells. Furthermore, immunostaining of the co-culture spheroids reveals the presence of vimentin-positive stellate cells and von willebrand factor (VWF)-positive sinusoidal endothelial cells. The spheroids also contain the activated stellate cell population marked by the smooth muscle actin (SMA) staining.
Kit Components:
| 3D Cell Culture Components | ||||
|---|---|---|---|---|
| Cat # | # of vials | Product Name | Quantity | Storage |
| 5000 | 1 | Human Hepatic Sinusoidal Endotdelial Cells (HHSEC) | 5 x 10^5 cells | Liquid nitrogen |
| 5300 | 1 | Human Hepatic Stellate Cells (HHSteC) | 5 x 10^5 cells | Liquid nitrogen |
| 3D-5201 | 1 | 3D-Hepatic Spheroid Medium (3D-HSpM) | 200 mL | 2-8 °C |
| 3D-5452 | 1 | 3D-Hepatic Stellate-Endothelial Spheroid Supplement (3D-HSteESpS) | 4 mL | -20 °C |
| 0583 | 1 | Penicillin/Streptomycin Solution (P/S) | 2 mL | -20 °C |
| 0343 (or) 0353 (or) 0383 | 2 | Ultra-Low Binding Culture Plates (24-, 48-, or 96- well plate) | 2 plates | RT |
| 2D Cell Culture Components | ||||
| Cat # | # of vials | Product Name | Quantity | Storage |
| 8711 | 1 | 2D-Hepatic Stellate-Endothelial Cell Co-culture Medium (2D-HSteECM) | 500 mL | 2-8 °C |
| 8762 | 1 | 2D-Hepatic Stellate-Endothelial Cell Co-culture Growth Supplement (2D-HSteECGS) | 5 mL | -20 °C |
| 0025 | 1 | Fetal Bovine Serum (FBS) | 25 mL | -20 °C |
| 0503 | 1 | Penicillin/streptomycin Solution (P/S) | 5 mL | -20 °C |
| 目录编号 | 3D-5000 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | 3D-HHSteECSF |
| 规格/数量 | 1 kit |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | Refer to product sheet. |
| 运输 | 5000、5300、3D-5452、0583、8762、0025 和 0503 使用干冰运输。3D-5201、8711 和（0343 或 0353 或 0383）常温运输。 |
