Cell Senescence Assay

Normal mammalian cells divide for a limited number of population doublings and eventually enter an arrested state in which the cells remain alive, but do not proliferate in response to mitogens, and assume a characteristic enlarged, flattened morphology. This process, termed senescence, is thought to be a tumor suppressive mechanism and underlying cause of aging. Senescence-associated β-galactosidase (SA- β-gal) is a widely used biochemical maker for assessing senescence in cultured cells. The ScienCell Cell Senescence Assay provide an easy-to-use method to detect SA- β-gal by staining cells with 5-bromo-4-chloro-3-indolyl- β-D-galactopyranoside (X-gal) at pH 6.0, a pH condition that suppress lysosomal β-galactosidase activity sufficiently to ensure that nonsenescent cells remain unstained.

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