This kit is a valuable tool for researchers studying the monkeypox virus, and we are proud to offer it to the scientific community.
What is the Monkeypox Virus?
Monkeypox virus (MPXV) is a zoonotic virus that can infect both humans and animals. It belongs to the Orthopoxvirus genus in the Poxviridae family.
The virus has two distinct genetic clades: the Central African clade and the West African clade. The Central African clade is considered more transmissible and causes more severe disease.
What is the ScienCell’s Monkeypox Virus Detection Kit (MPXVD, #RU7178) Kit?
The MPXVD kit is designed to detect the presence of the monkeypox virus in various sample types, including dry swabs, wet swabs, cell lysates, body fluids, and other biological specimens. The kit includes all the necessary components for DNA extraction, qPCR amplification, and result interpretation, making it a comprehensive solution for monkeypox virus detection.
Key Features of the MPXVD Kit:
- Detects Both Clades: The kit utilizes two primer/probe sets (MP1-FAM and MP2-FAM) that target highly conserved regions of the monkeypox virus, enabling the detection of both the Central African and West African clades. This broad coverage ensures accurate detection regardless of the circulating strain.
- High Sensitivity and Specificity: The carefully designed MP1-FAM and MP2-FAM primer/probe sets specifically recognize both clades of MPXV without interacting with other Poxviridae family viruses, such as vaccinia, variola, and cowpox viruses. This specificity minimizes the risk of false-positive results.
- Built-in Quality Control: The kit includes a human B2M gene target (HR-HEX) as a control to assess the quality of the specimen. This ensures reliable results and helps identify potential issues with sample collection or processing.
- Non-infectious Positive Control: A non-infectious positive control consisting of viral DNA fragments spiked into human dermal fibroblast cells is included to ensure reagents and instruments are functioning correctly. This control provides a benchmark for positive results and adds an extra layer of confidence to your experiments.
Components of the MPXVD Kit:
- Cell Lysis Buffer and Enhancer (Cat #GQ400a and GQ400b): These components are specifically designed for efficient DNA extraction from dry swab specimens.
- SapphiNStart TaqProbe qPCR Master Mix (Cat #MB6048a-1): This ready-to-use master mix contains all the necessary components for qPCR amplification, simplifying the workflow and reducing the risk of contamination.
- Nuclease-free Water (Cat #MB6048b-1): This high-quality water ensures optimal performance of the qPCR reaction and prevents interference from nucleases.
- Multiplex Primer/Probe Sets (Cat #7178-MPP): This component includes the three primer/probe sets (MP1-FAM, MP2-FAM, and HR-HEX) required for the detection of monkeypox virus and assessment of sample quality.
- Non-infectious Positive Control (Cat #7178-Pos): This control consists of non-infectious viral DNA fragments and serves as a reference for positive results, helping to validate the performance of the assay.
The MPXVD kit is a powerful and reliable tool for researchers studying the monkeypox virus. Its ability to detect both the Central African and West African clades, along with its high sensitivity and specificity, make it an invaluable asset in studying this disease.
Contact us to discuss your specific needs and discover the ideal ScienCell solution for your research!