Hepatic stellate cells have recently gained a great deal of attention regarding their contribution to the progression of diseases such as liver fibrosis, non-alcoholic steatohepatitis (NASH), and hepatocellular carcinoma. They… Read more »
qPCR is a powerful tool for quantification of gene expression levels and copy number variation. Despite the advances in next-generation sequencing (NGS), qPCR still serves as the “gold standard” for… Read more »
Cancer immunotherapy is one promising cancer treatment option whereby the host’s own immune system is used to treat cancer. The therapy works by either stimulating certain immune activities, or counteracting… Read more »
Aging and Telomere Length Quantification by qPCR Aging is a time-dependent decline of the body’s functional capabilities and is an inevitable course of life (as shown in the image… Read more »
The 2017 Nobel Prize in Physiology or Medicine was awarded to Jeffrey C Hall, Michael Rosbash, and Michael W Young for research that established key mechanistic principles on how circadian… Read more »
qPCR is still considered the gold standard for gene expression analysis, especially when template copy number is low. In short, NGS provides a great tool for analyzing a large number of genes to narrow down the research interest and qPCR provides a fast, reliable, and cost effective means to verify NGS results.