pNPP Phosphatase Assay

PNPP is suitable to detect acid and neutral phosphatase activity. The Assay Buffer-acidic (Cat #8108a), with a pH of 5.5, is optimized for acid phosphatase activity. The Assay Buffer- neutral (Cat #8108b), with a pH of 7.2, is compatible with most neutral phosphatases, such as protein phosphatases. PNPP is used to evaluate phosphatase activity in vitro and is for research use only. It is not approved for human pH 4-7 or animal use or for applications in clinical or in vitro diagnostic procedures.