超氧化物歧化酶活性检测试剂盒

Superoxide dismutase, as one of the body's most important defense mechanisms against freeradical damage, catalyzes the dismutation of the superoxide radical (O2-) into hydrogen peroxide (H2O2) and elemental oxygen (O2). In ScienCell's Superoxide Dismutase Assay (SOD), the superoxide anions, generated from the conversion of xanthine to uric acid and hydrogen peroxide by xanthine oxidase (XOD), reduce WST-1 (4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate sodium salt) to water-soluble formazans, which can be measured by absorbance at 438 nm. SODs lower the rate of the reduction reaction by reducing superoxide anion concentrations. Therefore, the % Inhibition of the reduction reaction can be determined as a measurement of SOD activity.

超氧化物歧化酶（Superoxide Dismutase, SOD）是机体抵御自由基损伤的重要防御酶之一，可催化超氧阴离子自由基（O₂⁻）歧化生成过氧化氢（H₂O₂）和分子氧（O₂）。

在ScienCell超氧化物歧化酶活性（SOD）检测试剂盒中，黄嘌呤氧化酶（XOD）催化黄嘌呤生成尿酸和过氧化氢的过程中产生超氧阴离子，该阴离子可将WST-1（4-[3-(4-碘苯基)-2-(4-硝基苯基)-2H-5-四唑基]-1,3-苯二磺酸钠）还原为水溶性甲臜类产物，其生成量可通过在438 nm处测定吸光度进行定量分析。

SOD通过降低体系中超氧阴离子的浓度，从而抑制上述还原反应的速率。因此，可通过计算反应抑制率（% Inhibition）来评估SOD活性。