Recombinant Protein A/G
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Protein G similar to Protein A, is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria but with different specificities. It binds to the constant region of many species of immunoglobulin G and can be used to detect, quantify and purify IgG antibodies and antibody/antigen complexes. Recombinant Protein A/G is an excellent tool for purification and detection of mouse monoclonal antibodies from IgG subclasses without interference from other serum proteins. Individual subclasses of mouse monoclonals are most likely have stronger affinity to this chimeric protein than to either Protein A or Protein G. Binding is less pH-dependent than either Protein A or Protein G alone. Optimal pH 5-8.
| Catalog No. | 1003-01 |
|---|---|
| Country of Manufacture | China |
| Product Code | rProteinA/G |
| Size/Quantity | 5mg, 20mg, or 1g |
| Product use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Upon reconstitution, store at 2-8 °C for up to one week. For maximal stability, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Shipping | Gel ice. Store at -20°C after receiving. |
| Synonyms | Protein A/G, r-protein A/G, protein a/g, protein-a/g, protein-ag, protein ag. |
| Source | Escherichia coli |
| Molecular Weight | Approximately 56.9 kDa, consisting of 6xHis-tag on C-terminus and 8 IgG-binding domains EDABC (Protein A)-C1C2C3 (Protein G). |
| Purity | > 95% by SDS-PAGE |
| Physical Appearance | White lyophilized powder |
| Formulation | Lyophilized from a 0.2µm filtered solution in PBS, pH 7.4. |
| Endotoxin | <0.1 ng/μg of protein (<1 EU/μg) |
| Reconstitution | Reconstitute in sterile distilled water or saline. |
No Publication available at this time.